Seeds used for Bodhi beads in China

Background Bodhi beads are a Buddhist prayer item made from seeds. Bodhi beads have a large and emerging market in China, and demand for the beads has particularly increased in Buddhism regions, especially Tibet. Many people have started to focus on and collect Bodhi beads and to develop a Bodhi bead culture. But no research has examined the source plants of Bodhi beads. Therefore, ethnobotanical surveys were conducted in six provinces of China to investigate and document Bodhi bead plants. Reasons for the development of Bodhi bead culture were also discussed. Methods Six provinces of China were selected for market surveys. Information was collected using semi-structured interviews, key informant interviews, and participatory observation with traders, tourists, and local residents. Barkhor Street in Lhasa was focused on during market surveys because it is one of the most popular streets in China. Results Forty-seven species (including 2 varieties) in 19 families and 39 genera represented 52 types of Bodhi beads that were collected. The most popular Bodhi bead plants have a long history and religious significance. Most Bodhi bead plants can be used as medicine or food, and their seeds or fruits are the main elements in these uses. ‘Bodhi seeds’ have been historically used in other countries for making ornaments, especially seeds of the legume family. Many factors helped form Bodhi bead culture in China, but its foundation was in Indian Buddhist culture. Conclusions As one of the earliest adornment materials, seeds played an important role for human production and life. Complex sources of Bodhi beads have different cultural and historical significance. People bought and collected Bodhi beads to reflect their love and admiration for the plants. Thus, the documentation of Bodhi bead plants can serve as a basis for future investigation of Bodhi bead culture and modern Buddhist culture.


Background
Humans use seeds (include the fruits with pericarps cannot be easily removed, commonly called 'seeds') in many ways. Seeds are colorful, durable, and easy to access, so humans have drilled and strung seeds into necklaces and bracelets for thousands of years. The oldest seed ornaments were discovered in Africa and can be dated to the Middle Stone Age (280,000 to 45,000 years ago) [1]. Moreover, due to the variety of medicinal and edible properties of plants and trees, people placed high value on this type of vegetation, and this feeling has continued to modern times [2]. Some people endow beads with specific meaning for averting disaster, developing wisdom, and soothing and relaxing.
Bodhi beads are Buddhist prayer items that have been traditional tools for counting while reciting a mantra, as prayer beads have been used in other world religions. The Bodhi beads are called Pu Ti Zi in Chinese: Pu Ti means Bodhi tree (Ficus religiosa), and Zi means seed; but Bodhi beads are not made of the seeds of a Bodhi tree (Ficus religiosa). The name Pu Ti Zi first appeared in the early medical text, 'Compendium of Materia Medica', which referred to the seeds of Coix lacryma-jobi [3]. In addition, seeds of Sapindus delavayi and Sapindus tomentosus were called Pu Ti Zi or 'Bodhi seeds' in Yunnan [4]. In modern times, 'Bodhi seeds' do not refer to any particular plant but instead refer generally to seeds and the fruits of various plants used to make prayer beads.
Bodhi beads have an important position in Tibetan Buddhism and are very popular in Tibet (the Xizang Tibetan Autonomous Region). The culture of Bodhi beads spread throughout Tibet and other provinces of China, and there are dozens to hundreds of Bodhi beads with different meanings. Recently, people have begun to wear Bodhi beads as a kind of praying or blessing ornament, in addition to functioning as a prayer bead. The Bodhi bead culture has developed in China in the form of cultural supplies, such as writing brushes and ink sticks. Several 'Bodhi seeds' of similar size, shape, and pattern are strung into strings of prayer beads. People frequently touch the beads with their hands to make the beads luster like jade due to secretion from human skin. Moreover, the beads are carefully modified to protect from scarring in inappropriate temperature and humidity surroundings.
Ethnobotanists study how plants are used for food and medicine but are also interested in plant adornments because non-mainstream use of plants can reflect relationships between cultures and (uses of) plants in other aspects. Surveys such as that conducted by Armstrong have shown that many botanical jewelries in different places were made by seeds and indicated the high value placed on the seeds [5,6]. Over 165 plant species used for human adornment in India were identified and listed [7].
Recently, there has been increasing interest in the commercial value of Bodhi beads, and many books about the culture of Bodhi beads have appeared in China. Although the books and related materials focus on the meaning of each kind of Bodhi bead, almost all publications ignore the question of which plant or plants produced the Bodhi seeds. The beads have been commonly described as 'seeds of rare plants', and this description has fostered a belief that the value of Bodhi beads is related to the scarcity of their sources and not to their aesthetic and cultural value. To help correct this misunderstanding, we investigated the plant sources of 'Bodhi seeds' or Pu Ti Zi and explored the culture values of Bodhi bead plants.

Study area
Surveys were conducted in local markets and e-commercial platforms of six provinces in China: Tibet, Yunnan, Fujian, Zhejiang, Beijing, and Guangdong. Field work was conducted from September to October 2012 and August to September 2013. Barkhor Street (29°39′ N, 91°7′ E) is a famous street circling Jokhang Temple in Lhasa, Tibet, and was the main local market surveyed. The traditional culture and local lifestyle have been well-preserved for more than 1300 years. We choose this market because of the wide variety of Bodhi beads traded there between vendors, pilgrims, and tourists from Nepal, Bhutan, India, and other places in China. It is the most important commercial center, folk-custom heritage, tourist attraction [8], and shopping center in Lhasa. Other Bodhi bead trading centers were also investigated, including Shangri-la and Kunming in Yunnan, Xiamen in Fujian, and cultural markets and temples in Beijing.

Data collection
Bodhi beads samples were purchased from Barkhor Street markets and e-commercial platforms. A detailed inventory and related information for the Bodhi beads were prepared from semi-structured interviews, key informant interviews, and participatory observation with traders, tourists, and local residents. In order to access the popularity of Bodhi beads in Chinese markets, various Bodhi beads in 126 shops of six provinces were studied. The number of shops that each Bodhi bead was sold in was counted for an occurrence frequency (OF) calculation; for example, if N shops sold Bodhi bead type ' A' (N ≥ 1), then the occurrence frequency of ' A' is OF(A) = N/126 × 100%.

Bodhi bead plant diversity and distribution
Fifty-two types of Bodhi beads were documented ( Table 1), made of seeds from 47 species (including 2 varieties) belonging to 19 families and 39 genera. Among them were only one endangered (EN) species (Latania loddigesii -'Xian-Zhi') in IUCN [25] and two critically endangered (CR) species (Dracontomelon macrocarpum -'Grimace' and Cycas revoluta -'Buddha-mind') in the China Species Red List [26]. The majority of Bodhi seed plants belonged to Arecaceae (12 genera and 13 identified species) and Fabaceae (7 genera and 8 identified species). The genera represented by the highest number of species was Caesalpinia (3 identified species). Most seeds of a particular species can produce only one type of Bodhi bead. However, different degrees of processing of Corypha umbraculifera, Daemonorops jenkinsiana, and Elaeis guineensis can be used to make different types of Bodhi bead. The seeds with 2-, 3-or 4-loculed drupes of Ziziphus abyssinica can be used to make 'Phoenix eye', 'Dragon eye', and 'Kylin eye', respectively. Chinese names of most Bodhi beads are according to the morphological characteristics of their respective seeds; for example, the beads called 'Moon and Stars' usually have an ivory surface with small holes (moons) and tiny black dots (stars). Seeds of Caesalpinia

Traditional cultural value of Bodhi bead plants The most popular Bodhi beads and their potential cultural significance
The occurrence frequencies of four types of Bodhi beads reached at least 50%, including 'King Kong', 'Moon and star', 'Bodhi root', and 'Phoenix eye' (Figure 1). These were the most popular Bodhi beads in the markets.
The most common Bodhi bead, 'King Kong', is made from the fruit of Elaeocarpus angustifolius. 'Two-furrowed King Kong' is made from the fruit of Elaeocarpus hainanensis. Elaeocarpus plants are distributed in Hainan, Yunnan, Guangxi, Tibet of China, and other parts of Asia, including Nepal, Bhutan, India, Indonesia, and the lowlands of the Himalayas [27]. 'King Kong' may have been the earliest form of prayer bead in India [28], named 'Rudraksha' in the local language, meaning 'eye of Shiva' [27]. The furrows of the hard and rugulose endocarp of fruits are important to the rudraksha. Different numbers of furrows represent different meanings. The rough surface symbolizes the austere life expected of worshippers [28]. Five furrows and up to 14 furrows are usual, while others are very rare [2]. Normally, a string of prayer beads is made with 32, 108, or 112 beads of similar size and the same number of furrowed rudraksha seeds. The standard 'King Kong' string has 108 Bodhi beads, and this string of prayer beads is widely used by Tibetan Buddhists [29].
'Moon and stars' is a very popular and traditional Bodhi bead in Chinese Buddhism and is the hard and dense seed of Daemonorops jenkinsiana. The 'Moon and stars' name reflects the small holes (moon) and tiny black dots (stars) covering the seed's surface. This species is mainly distributed in the south of China, India, Nepal, Bhutan and Bangladesh. There was no record of the history of this type of Bodhi bead, but we found many old 'Moon and stars' beads in Tibet; we therefore speculated that Tibetan Buddhism was influenced by mainland China's Buddhism.
Seeds of Corypha umbraculifera are used to make 'Bodhi root' beads. This species is native to India and Sri Lanka. In India, it is also called 'vegetable ivory' and is a traditional tool for carving Buddhist Sutras, such as the famous 'tale palm' or 'tad-patri' because the leaves are flexible and soft when dry [30][31][32]. Because of early Indian Buddhist influences [33], C. umbraculifera was cultivated in temple gardens in Xishuangbanna of Yunnan Province in China, and over 50,000 volumes of Buddhist Sutras carved on leaves were protected there [34]. In addition, ancient books and letters of Southeast Asian countries were written on its leaves [35]. Therefore, C. umbraculifera is regarded as one of the iconic Buddhist plants in Asia. 'Phoenix eye' and 'Small phoenix eye' are made from the fruits of Ziziphus abyssinica and Ziziphus jujuba var. spinosa, respectively. The name 'Phoenix eye' refers to the eye-like shape that appears on the hard endocarp. Z. abyssinica is native to India and is not cultivated in China. Both were used to make adornments in India [7,36]. Ziziphus has been mentioned more than one time in the Ramayana and the Mahabharata [37]. The tree and fruit of this genus have great significance in Indian traditional culture. Jujube trees are one of the few sacred trees of the Sikhs in India. The species of jujube tree named Christ's Thorn Jujube (Z. spina-christi) is the only tree that could be regarded as a holy tree in Islam [38]. Species of Ziziphus are also held sacred by many other religious persons, such as some Druze, Muslims, and Christians.

Traditional playing nuts in China
Chinese olives (Canarium spp.) have a long history as accessories in China. Drupes of Chinese black olives (Canarium pimela) are the main material for carving and playing and are produced mainly in the Chinese provinces of Guangdong and Fujian. 'Olive-stone carving' prospered in the Ming to Qing (1368-1911) dynasties, although it might have originated earlier. The most classical 'olive-stone carving' is the 'Olive nut boat.' Playing with walnuts is a traditional Chinese practice with its origin in traditional Chinese medicine therapies: the Chinese consider the bumps and sharp edges of a walnut to promote blood circulation when playing with walnuts in one's hands [39]. Each Juglans species in China can be used for playing with walnuts, including Juglans regia.

Similar uses of Bodhi bead plants in different countries
Eighteen species presented in the present paper were also used for making ornaments in other countries. Seeds of 12 species were strung for necklaces and strings of prayer beads in India. In addition to E. angustifolius, C. umbraculifera, Z. abyssinica, and Z. jujuba, plants used as adornment in India include Thevetia peruviana, Abrus precatorius, Areca catechu, Coix lacryma-jobi, Adenanthera pavonina, Caesalpinia bonduc, Entada phaseoloides, and Melia azedarach. Ten species were used in the American continent, as well. Most of these species were used as a simple necklace without religious meaning. Seeds of A. precatorius were widely used because of their strikingly beautiful appearance and amazing durability. The world-famous 'plant elephant' is the seed of Phytelephas macrocarpa and is a type of precious Bodhi bead in China named 'Ivory nut.'

Multiple uses of Bodhi bead plants in China
In addition to making Bodhi beads, all Bodhi bead plants in China have other uses. Thirty-six species (76.6%) are also used as medicine. The seeds or fruits of most species are the main medicinal parts. Some species can be used to treat heart disease, dyspepsia, hypertension, rheumatism, and other ailments. Except for Mucuna gigantea, all seeds of the legume family used to make Bodhi beads have medicinal functions. For example, seeds of Abrus precatorius, Acacia confusa, and Adenanthera pavonina can be used to treat skin diseases. Seeds of Afzelia xylocarpa have an anti-inflammatory function, while seeds of Caesalpinia bonduc or C. minax can be used to treat rheumatism [40]. Although there was no record about whether C. major was cultivated in China, it has been recorded as a traditional Chinese medicine [41]. Twenty species (42.6%) of Bodhi bead plants are also used as landscape plants or for aesthetic purposes, especially the species in Arecaceae. Seeds of these plants are the main sources of materials for making Bodhi beads. Most of the Arecaceae species are cultivars introduced into China and were mainly cultivated as ornamental plants in parks, scenic spots, or roadsides. Five species in Arecaceae are important fiber plants (10.6%). The stem of Daemonorops jenkinsiana can be used for furniture-making and is an important source of rattan material in South China [12]. Leaves of Corypha umbraculifera can remain flexible for a long period and can be used for making fans, mats, umbrellas, baskets, thatching, roofing, and so on [32]. Sixteen species of Bodhi bead plants (34.0%) are edible. The main edible parts are their seeds or fruits, such as Dracontomelon macrocarpum and Ziziphus jujuba var. spinosa. The central soft part of the stem of C. umbraculifera is a rich source of starch that can be made for sago. The most valuable commercial Bodhi bead plants are four oilbearing species and nine timber species. Elaeis guineensis is called oil palm and is the main oil-bearing crop in tropical areas. The oil content of its fruit is higher than 50%, and this species has the highest productivity of any oil-bearing plant in the world [42].

Conclusion
As one of the most popular Buddhist supplies, Bodhi beads are being paid more attention and becoming the target of many collectors. However, the sources of Bodhi beads have been ignored.
The purpose of the current study was to give an account of Bodhi bead plants and the reasons for the widespread use of Bodhi beads in China. Seeds of 47 species (2 varieties) were recorded to be used for making 52 types of Bodhi bead. Some of these seeds were also used in other countries as necklaces or strings of prayer beads. They might be the oldest materials used for ornaments. Twelve of them were traditional Indian beads, especially rudraksha seeds. Together with other items, they were the earliest prayer beads in Indian Buddhism. We also found that all of the most popular Bodhi bead plants had a close historical relationship with religion. Bodhi bead plants have multiple uses in China. Besides aesthetic functions, they can be used as food, medicine, timber, and plant oil. These seeds and other parts of plants played a prominent role in the lives of humans for thousands of years. Bodhi beads might be the byproducts of plants, and use of seeds for Bodhi beads might have increased the conservation value of these plants and helped biodiversity conservation in China. The evidence from this study suggests that Buddhism culture combined with Chinese collection culture resulted in modern Bodhi bead culture and that commercial interest in Bodhi beads promotes the spread of Bodhi bead culture.