Wild leafy vegetables: A study of their subsistence dietetic support to the inhabitants of Nanda Devi Biosphere Reserve, India

Consumption of greens is a major source of vitamins and micro-nutrients for people using only vegetarian diets rich in carbohydrates. In remote rural settlements where vegetable cultivation is not practiced and market supplies are not organized, local inhabitants depend on indigenous vegetables, both cultivated in kitchen gardens and wild, for enriching the diversity of food. Knowledge of such foods is part of traditional knowledge which is largely transmitted through participation of individuals of households. A total of 123 households in six villages of Nanda Devi Biosphere Reserve buffer zone was surveyed using a schedule to assess the knowledge, availability and consumption pattern of wild leafy vegetables. Quantity estimations were done using regular visits with informants from 30 sample households of the six study villages during the collections. Monetization was used to see the value of wild leafy vegetables harvested during a year. The diversity of wild leafy vegetables being use by the local inhabitants is 21 species belonging to 14 genera and 11 families. This is far less than that being reported to be used by the communities from Western Ghats in India and some parts of Africa. Irrespective of social or economic status all households in the study villages had the knowledge and used wild leafy vegetables. The number of households reported to consume these wild leafy vegetables is greater than the number of households reporting to harvest them for all species except for Diplazium esculentum and Phytolacca acinosa. The availability and use period varied for the species are listed by the users. The study indicated that the knowledge is eroding due to changing social values and non participation of younger generation in collection and processing of such wild leafy vegetables.


Background
Since time immemorial useful plants have been handled by human societies for medicinal and food purposes. While, the hunter-gatherer societies still continue to profess such lifestyles, the agricultural societies did not eliminate the use of non-cultivated resources. Today, most human plant food is based on rather limited number of crops (12 crops contribute more than 85-90% of worlds caloric intake), but it is clear that in many parts of the world the use of wild plants is not negligible [1][2][3][4][5][6][7]. Diet surveys tend to ignore wild plants in comparison to cultivated ones [8], and this is a methodological deficiency [9].
The need for conservation of genetic resources, mostly those of wild relatives of crop plants, which can be useful in case of genetic erosion or for crop improvement, is the driving force behind our interest of studying the wild food plants [10,11]. Changing social values, depopulation of rural areas has led to erosion of traditional knowledge [12,13]. Many publications [10,11,[14][15][16][17][18][19][20][21][22][23]28] have emphasised on the diversity and value of traditional vegetables. The nutritional value of traditional leafy vegetables is higher [18,[24][25][26] than several known common vegetables. Most of these traditional leafy vegetables have a potential for income generation but fail to compete with exotic vegetables at present due to lack of awareness [ [12,15] and [27]]. Consumption of traditional diets known to these societies are said to have many beneficial effects such as prevention of some age related degenerative diseases -arteriosclerosis, stroke, etc. [13]. Despite these advantages, most traditional plant foods are generally uncultivated and underutilized [5,22].
Uttarakhand (20° 26' and 31° 38' N latitude and 77° 49' and 80° 6' E longitude), a province (state) in India, covering an area of 53,483 km 2 and with the population density of 159 persons/m 2 is rich in diversity of wild edibles [14,17]. Nanda Devi Biosphere Reserve (NDBR), a world heritage site, occupies a special place in the biosphere reserve system of higher Himalayan region of India (Figure 1). Tolchha and Marchha sub communities of Bhotiya tribe are the main inhabitants of Niti and Mana Valleys which form buffer zone of NDBR. Besides, these traditional communities and non-tribal Khasa group also inhabited these two valleys. There are 419 households with 2253 individuals during 2001 census [29]. The sex ratio of the population is 919 and the total literacy is 36.7% [14]. Based on the distance from core zone the area Nanda Devi Biosphere Reserve and study locations Figure 1 Nanda Devi Biosphere Reserve and study locations. could be broadly segregated into three main elevation zones i.e., Higher (2800-3600 m), middle (2400-2800 m) and lower (1900-2400 m) ( Table 1). The four villages (Malari, Dronagiri, Garpak and Niti) representing higher elevation zone has maximum arable land where only one crop is grown in a year and the people practiced traditional transhumance. Middle elevation zone is represented by five villages (Tolma, Suki, Bhallagaon, Phagti and Laung) with least arable land, 3 crops in 2 year cropping cycle and the households practice short migration (modified transhumance). The lower zone is represented by three villages (Lata, Reni and Peng) where 3 crops in 2 years are cultivated and practice permanent settled agriculture on rainfed terraces. The number of cultivated crops decreased with increase in elevation. Predominant land use in these valleys is forests and alpine grazing lands. All inhabitants practice permanent cultivation of terraced slopes. Total cultivated area is 273 ha and about 8150 domestic animals are recorded in these villages. In addition to agriculture, about 11% of households have one or two members of the household involved in business and about 31% of households have one or two members in government or semi-government employment.
The climatic year consists of three distinct seasons -summer (April-June), rainy (June-September) and winter (October-February). Average annual rainfall is 928.81 mm and about 47% of this occurred during July and August itself. The mean monthly maximum and minimum temperatures varied between 24-14°C and 7.5-3°C, respectively [16]. Parent material is crystalline rocks, includes garnetiferous mica schists, garnet mica quartz schists and mica quartzite. The soils in general are deep in agricultural land, black in colour, loam to sandy loam and well to excessively drained.
The Nanda Devi Biosphere Reserve is one of the most biologically diverse areas of the western Himalaya. Though, the area lies in high altitude region, the high degree of variation in elevation resulted in diversity of microhabitats with a number of unique vegetation types. This unique feature acted both as a bridge, facilitating the influx of many taxa, and as a barrier, promoting endemism in the area. The reserve is a repository of a large variety of medicinal plants and animals having economic value. However, due to over exploitation, populations of a number of plant and animal species have become low in their natural habitats [30,31]. These species have now become rare, endangered and threatened. Some of the important taxa that are listed as threatened are Aconitum heterophyllum Wall ex. Royle, Podophyllum hexandrum Royle, Dactylorrhiza hatagirea D.Don, Nardostachys grandiflora DC. and Taxus buccata L. Similarly, several faunal elements such as snow leopard, Himalayan brown bear, musk deer, monal pheasant, Himalayan snowcock and snowpatridge are also said to be endangered.
The core diet of the inhabitants of the region is rice, wheat, pulses and a wide variety of local wild and semi-domesticated plants. Protein requirements are supplemented with animal products such as milk and meat. Most households grow domesticated vegetables in their kitchen gardens. Wild foods are considered by the local inhabitants in the region as necessity rather than as a supplement and are eaten frequently [14]. While several studies were conducted to document the diversity of resources [30,31] and their ethnobotanical uses [16,17,32], very few studies prioritized the species of local importance and quantified their availability, use pressure and method of use [11]. The purpose of the present study was to document plant species consumed as traditional and leafy vegetables and their ecological biodiversity in a world heritage site Nanda Devi Biosphere Reserve, India. In this study we attempted to prioritize the leafy vegetables extracted from wild and documented their status and consumption. was used to collect information on personal data, traditional knowledge and priority rank for each species listed by the household. Adult female member from the household, who is responsible for food preparation, was considered as the respondent with additional information from children and adults (those assisting in collection and processing of wild leafy vegetables). Field visits were made with the informants for collection of specimens. Identification of the collected specimens was made with the help of flora of Chamoli [36] and a botanical work of the Nanda Devi National Park [30]. Herbarium specimens of the Department of Botany, H.N.B. Garhwal University, Srinagar (Garhwal) were also consulted. Prioritization of the leafy vegetables was done using a set of criteria considered to be main drivers of consumption and their availability in wild. These criteria are: (i) palatability (good, medium and low), (ii) medicinal use (yes and no), (iii) frequency of occurrence in the natural habitats (rare, intermediate and frequent), (iv) quantum of extraction (large, medium and small) and (v) existence of market value (yes and no). Based on the responses given by the 123 households surveyed, nine species either reported to be rare in occurrence or large in quantum of extraction were shortlisted. Following species satisfied these criteria viz. "doom" (Allium semnovii Regel), "bethua" (Chenopodium foliolosum Hook.), "lingra" (Diplazium esculentum (Retz.) Sw.), "dhol kanali" or "pachu" (Girardinia diversifolia (Link.) Friis.), "barmau" (Megacarpaea polyandra Benth.), "chandra" (Paeonia emodi Wall ex. Royle.), "jagra" (Phytolacca acinosa Roxb.), "payoom" (Rumex nepalensis Spreng.) and "puyanu" (Smilacina purpurea Wallich). "Jangli chaulai" (Amaranthus bilatum L.) was also prioritized for the study as we were surprised to see large extraction pressure on this genus occurring in wild though most families also cultivated the domesticated amaranth. Five families in each village were selected in January 2004 for monitoring the number of visits to collecting places, quantity of resource extracted, details of use and mode of consumption. Selection of these households involved some amount of bias as households with females having basic literacy and ability to fill in the details in data sheets was used as a criteria. This was required as the research team could not be present in all the villages at all times. However, during the visits to each of these villages, the research team personally accompanied the informant to the fields to document the extraction, processing and preparation. Using the information supplied by the informants the amount of wild leafy vegetables harvested by each family is estimated. The mean value is used to derive the total of the six villages.

Results
Twenty-one wild plant species belonging to 14 genera and 11 families are identified as being used as leafy vegetables by the informants from 123 households surveyed (Table  3). Only one plant belongs to Pteridophyta and all others to Magnoliaphyta. Two genera and five species belong to monocots (Liliopsida) and remaining are dicots (Magnoliopsida). The period of collection started from February and continued till the end of October. For most species it is short (30-60 days) but for some species such as Chenopodium foliolosum, Phytolacca acinosa and Allium spp. it is 120 days and for Rumex spp. it is 240 days. While palata- bility of most species is medium or low, for Allium spp., Amaranthus bilatum, Chenopodium foliolosum and Megacarpaea polyandra it is good and more frequently used as leafy vegetable. Except Amaranthus bilatum, Chenopodium foliolosum and Diplazium esculentum all others had medicinal uses also. Dried fronds of Diplazium esculentum are reported to be preferred animal bedding material during winters. Several of these species have market value, but only Diplazium esculentum and Megacarpaea polyandra are actually sold or purchased as fresh vegetables in the study villages. All other species with market value are sold or purchased as medicines in dried or processed forms both in the study area and exported outside. Among all the leafy vegetables only Fagopyrum debotrys is reported to be rare and mostly occurring in alpine pastures. Though it is reported to be rare we did not have opportunity to quantify its extraction as our sampling was restricted to settled villages only.
Megacarpaea polyandra, Paeonia emodi and Smilacina purpurea are processed for storing to be used as vegetable during periods of non-availability in addition to their consumption as fresh vegetable ( Table 4). None of the respondents reported that a visit was made exclusively for collection of leafy vegetable from the wild. It is common for the households to collect these leafy vegetables during their visits to various places such as grazing lands, forest, crop fields and watercourses for grazing the animals, collecting fuel or fodder, tending the crop fields or collecting water etc. None of these leafy vegetables required any special processing for cooking or consumption, though removal of stings from nettle and hairs from fronds of vegetable fern could be specific requirements. All the leafy vegetables are prepared like spinach and eaten as a form of stew or cooked in oil (mainly mustard oil which is the preferred cooking medium in the study area) with salt and spices.  Generally grows under the canopy of Betula utilis L and Abies pindrow L trees in forests. Due to excessive collection pressure becoming rare in nature, but some villagers have started cultivation in kitchen garden.
Fresh leaves are boiled or fried in cooking oil with spices. Leaves are smoked by hanging them above cooking stoves and then stored for consumption during winters.
Chandra Paeonia emodi Wall. ex. Royle. Generally grows in alpine grazing lands and forests near moist areas where Juglans regia L or Populas deltoides L is dominating.
Fresh leaves are boiled with spices. Cooked leaves are fermented and preserved as a leaf cake for lean period consumption. Jagra Phytolacca acinosa Roxb.
Commonly grows near forest margins and on agricultural terrace raisers.
Fresh young leaves are collected and used only during March as mature leaves are said to have poisonous substances. Fresh leaves are boiled, mashed and fried in cooking oil with spices. Payoom Rumex nepalensis Spreng.
Commonly grows near to water sources. Fresh young leaves are boiled or fried in cooking oil with spices. Puyanu Smilacina purpurea Wallich.
Commonly grows under Betula utilis L and Abies pindrow L forests.
Fresh leaves are boiled or fried in cooking oil with spices. For lean periods, leaves are air dried and the smoked by keeping the dried leaves in an earthen pot hanging above cooking area. of this plant is expanding in the region. The number of households collecting a specific leafy vegetable is highest (90%) for Megacarpaea polyandra followed by Amaranthus bilatum (85%) and Allium semnovii (65%), Diplazium esculentum (65%). Girardinia diversifolia is used by least number (25%) of the households. This species is also reported to be used for preparation of a snack in addition to being used as vegetable. Phytolacca acinosa is reported to be used by 45% households although its use is restricted to less than 30 days due to harmful constituents in old leaves. The number of households consuming the wild leafy vegetables is greater than the number of households reporting to collect them except for Diplazium esculentum and Phytolacca acinosa.

Discussion
The benefits of wild resources to inaccessible rural villages in Himalaya cannot be ignored. The positive relationship between the resources i.e., crops, non timber forest products and livestock indicate their concurrent relevance to livelihoods [37,38]. The number of wild leafy vegetables recorded in the present study area indicates its diversity is less as compared to other areas [26,39]. According to several informants wild green leafy vegetables increase the amount of blood in the body which is likely to refer to the high iron content of many wild greens. However, chemical analyses were beyond the scope of this study, and therefore, the information on the nutrient contents is entirely based on literature. The majority of wild edible herbs eaten typically contain high levels of important nutrients especially for diets usually high in starch [18,22,[36][37][38][39][40][41][42][43][44][45]. The informants who reported these uses know perfectly well that these plants can be noxious, but they only ate some very particular parts of the plant or they use them in very small quantities. The case of Phytolacca acinosa in the study area elucidates the knowledge of toxicity. Such knowledge is common in other areas too [26,39,46,47]. Whilst the herbs are eaten as leafy vegetables, the majority does play an opportunistic or overlapping role as medicinals [14,16], and hence adding extra value, and thereby making them very attractive and important to the users. Wild leafy vegetables prepared as curry are eaten with bread made of ground wheat/barley/ finger millet. Wild edible herbs provide important leafy vegetables for many rural households [14]. Households with limited access to cultivated vegetables such as the present region had to store dried herbs for use during the lean periods. Households that consumed herbs daily were by far the majority. This emphasizes the role of herbs in the diets of people, similarly reported in other studies [9,11,18,23,26].
There were no differences between the numbers of households that harvested wild edible herbs from microenvironments within and around the village, indicating the importance of the full range of environments. However, whilst herbs are collected from a range of environments, the amounts collected from village commons tended to be higher than that collected from distant alpine rangelands. Therefore, households do recognize all these units of land as important sources of the species they harvest and may contribute cultural significance to any management options designed for these lands. The information collected in the present study did not show any association between the income or social status and use of wild edible herbs. Households with financial means to purchase cultivated alternatives also reported that they consumed the wild edible herbs. This demonstrates the strong cultural underpinnings of the use of wild edible herbs [48] although the remoteness of the village from markets that supply cultivated species must have a role to play [49]. We did not notice any differences in preferences for wild edible herbs between households in a village. Though our data collection methods do not permit us to do any analysis, our observations during data collection clearly indicated that the knowledge about the plants is more common with older people (>35 years) as compared to young adults (13-25 years). Some of the respondents even commented that the young adults are not participating in collection and processing of these wild leafy vegetables and thus the knowledge about some of the species may disappear. This was also reported by other workers [9,39] from elsewhere. We recognize the need for collecting, preserving and documenting this knowledge as an urgent and fundamental necessity not only for maintaining the local cultural traditions but also to facilitate the research on new food sources elsewhere as well.
About two-thirds of households that consumed wild edible herbs indicated that there were sufficient herbs available for harvesting in the year of the study. However, when residents were asked to compare the current availability with the past decade, the majority was in agreement that the amount of wild herbs has decreased. This is not surprising because several households cultivated some of the wild herbs now [14]. The marginal lands and traditional agroecosystems are important sources of wild leafy vegetables as observed in this study and can make important contributions to biodiversity conservation and food security. Wild edible herbs in traditional communal areas of NDBR often grow on lands that extend from the immediate neighborhood of the built area of human settlement to the arable lands and grazing areas and at times in disturbed areas. There is also great economic potential of some species upon analyses of their nutritional and chemical composition based on species popularity and importance [28]. The high degree of coincidence of food and medicinal uses of most of these plans is particularly remarkable. In the frame of an increasing interest on pharmafoods or nutraceuticals [8,12], the study of non-crop food plants may be useful in the development of such products.

Conclusion
Traditional knowledge is a tacit or an implicit type of knowledge that people know and apply but do not normally express. Moreover, it is localized because it gains a particular place only through experience and practice by a particular community environment. It is also dynamic and changes in the context. The way this knowledge is transmitted in not institutionalized and thus is not taught through conventional education systems. This means that traditional knowledge requires participation of individuals for learning and thus is vulnerable to loss in the face of environmental and societal changes in the rural domains. The monetization of wild edible herbs, which are mostly without formal markets, is key to understanding their value to locally unrecognized economies and hence the networks of strategies used mostly by households in remote areas for their livelihoods [50]. The study shows that wide ranges of uncultivated species are used by the majority of households as leafy vegetables. These herbs are harvested and used directly (i.e., direct-use value), by the households and often without any form of trade especially because the majority of the households often engage in the harvesting within their local environments. The direct-use value therefore represents a reasonable replacement cost for the cultivated alternatives, and offers extra cash savings for the household. Value addition through storage and commercialization could probably widen the livelihood base and thus draw attention of planners. Such strategies have been effectively used to combat vitamin and micro-nutrient deficiencies in Africa [51], and thus should be replicated in all other regions.