Ethnomedicinal study of plants used in villages around Kimboza forest reserve in Morogoro, Tanzania

Background An ethnomedicinal study was conducted to document medicinal plants used in the treatment of ailments in villages surrounding Kimboza forest reserve, a low land catchment forest with high number of endemic plant species. Methods Ethnobotanical interviews on medicinal plants used to treat common illnesses were conducted with the traditional medical practitioners using open-ended semi -structured questionnaires. Diseases treated, methods of preparation, use and habitat of medicinal plants were recorded. Results A total of 82 medicinal plant species belonging to 29 families were recorded during the study. The most commonly used plant families recorded were Fabaceae (29%), Euphorbiaceae (20%), Asteraceae and Moraceae (17% each) and Rubiaceae (15%) in that order. The most frequently utilized medicinal plant parts were leaves (41.3%), followed by roots (29.0%), bark (21.7%), seeds (5.31%), and fruits (2.6%). The study revealed that stomach ache was the condition treated with the highest percentage of medicinal plant species (15%), followed by hernia (13%), diarrhea (12), fever and wound (11% each), and coughs (10%). Majority of medicinal plant species (65.9%) were collected from the wild compared to only 26.7% from cultivated land. Conclusions A rich diversity of medicinal plant species are used for treating different diseases in villages around Kimboza forest reserve, with the wild habitat being the most important reservoir for the majority of the plants. Awareness programmes on sustainable utilization and active involvement of community in conservation programmes are needed.


Background
Kimboza forest reserve has 13 recorded endemic plant species making it the richest lowland forest in East Africa. The forest reserve has valuable contribution to biological and gene pool conservation, and together with other mountain ranges of Morogoro region form part of the Eastern Highlands of Tanzania with about 200 endemic plant species [1,2]. The uses of plants in the indigenous cultures particularly of developing countries, are numerous and diverse, forming an important socio-economic base including their use as medicine [3]. People generally depend on nearby forests for fuel wood, timber and medicine. Medicinal plants therefore have important contribution in the primary healthcare systems of local communities as the main source of medicines for the majority of the rural population [4,5].
The World Health Organisation (WHO) estimates that up to 80% of the world's population in developing countries depend on locally available plant resources for their primary healthcare, since western pharmaceuticals are often expensive, inaccessible or unsuitable [6]. Further, in this decade, the world is experiencing an increasing rate of resistance by pathogens to some of the synthetic drugs, as well as the struggle against some chronically complex and uncontrolled infections such as Cancer and HIV/AIDS. There is therefore need to study and validate ethnomedicines for wider acceptance, recognition and utilization by all stakeholders in the society. However, overtime, ethnomedicinal knowledge has been undermined by mortality of resource persons, extinction of plant resources, inadequate scientific research and poor documentation [7]. The aim of the present study was therefore to document ethnomedicinal information of plants used by indigenous people in villages surrounding Kimboza forest reserve. The generated information will be used in future to explore ways of sensitizing the community on the sustainable utilization of the forest resources so as to minimize their genetic loss.

Study area
The study area is about 60 km from Morogoro Municipal located between 06°59' -7°02' S and 37°47' -37°4 9'E. An ethnobotanical survey for medicinal plants was conducted in the following villages: Changa, Kibangile, Mwarazi and Uponda which surround Kimboza forest reserve ( Figure 1). The communities adjacent to the forest have access right over the forest as stipulated in the village forest management plan by-laws.

Data collection
Ethnobotanical surveys were carried out to obtain information on medicinal plants traditionally used to treat various ailments in the study area. With the help of local administrative officers, Participatory Rural Appraisal (PRA) was employed to identify key respondents [8]. Ethnobotanical data was collected through individual and Focus Group Discussion (FGD) interviews using semi-structured open-ended questionnaires. Interviews were conducted in Swahili or the local Kiluguru language. Field excursions were also conducted with the assistance of key respondents. Information regarding the local names of the plant species, medicinal uses, parts used, methods of preparation, and administration route were documented. The methods used in harvesting the plant materials from the wild were also recorded. Plant specimens were prepared, identified and voucher specimens deposited in the University of Dar es Salaam herbarium for future reference. Descriptive statistics were used to analyze the ethnomedicinal data collected.

Results and discussion
A total of 22 traditional medical practitioners were interviewed, with their ages ranging between 28 to 70 years, and 60% of them being older than 50 years. Out of the 22 informants interviewed, 18 of them were men and only four were women. A total of 82 medicinal plant species in 29 families were recorded as being used to treat 41 different types of diseases as shown in Table  1. The family Fabaceae had the highest proportion of medicinal plants used (29%), followed by Euphorbiaceae (20%), Asteraceae and Moraceae (17% each), and Rubiaceae (15%) in that order ( Figure 2). Each of all other families had less than six plant species associated with the treatment of the diseases documented in Table 1.
At least 65.9% of all medicinal plants recorded were collected from the wild while only 26.7% were collected from cultivated land. The most frequently utilized medicinal plant parts were leaves (41.3%), followed by roots (29.0%), bark (21.7%), seeds (5.3%), and fruits (2.6%) as shown in Figure 3. Roots were mostly used in the treatment of stomachache, diarrhea and inflammatory diseases, while leaves were mostly used in the treatment of malaria, skin diseases and children's diseases.
Most of the plant species were used to treat one disease, while some were used to treat two or more diseases. The plant species used to treat the highest percentage of diseases were Azadirachta indica A. Juss. and Bridelia micrantha (Hochst) Baill. each reported to treat 14.6% of the diseases. Ficus exasperate Valh., Mangifera indica L. and Senna hirsuta (L.) Irwin & Barneby. were each reported to treat 12.2% of the diseases. The third category of highly used plant species were Ocotea usambarensis Engl. and Vernonia hymenolopis A. Rich. each reported to treat 9.8% of the diseases (Figure 4). In terms of frequency of medicinal plant uses, the highest percentage of plant species (15%) was used to treat stomachache, followed by diarrhea (13%) and hernia (12%), fever and wound (11% each) and cough (10%). Other diseases were treated with less than 10% of the medicinal plants recorded ( Figure 5).
Majority of the plant remedies were prepared by boiling (44%), then by crushing or pounding (27%), or soaking in cold water (19%) and concoction 10% ( Figure 6). Some specific herbal preparations were taken by mixing       with food, honey or drunk together with coffee prepared from leaves of the coffee plant. Most medicinal plant preparations were taken orally (75.6%), while 24.4% were administered topically for diseases such as skin infections and wounds. It was reported that different dosages were given to similar disease conditions. Administration dosage was by estimation and the amount of each dosage depended on the age of the patient and severity of the condition being treated.   The study revealed a rich diversity of medicinal plants used to treat various disease conditions in the villages around Kimboza forest reserve. It further revealed a rich ethnobotanical knowledge amongst the residents of the neighbourhood of Kimboza Forest reserve. The families Fabaceae, Euphorbiaceae, Asteraceae, Moraceae and Rubiaceae which were the most dominant in this study are consistently recorded in other ethnomedicinal studies [9][10][11][12], and could be attributed to their wide range of bioactive ingredients [13,14].
The fact that majority of the plant species recorded were sourced from the wild and only a few were cultivated may imply that many indigenous plant species may be difficult to propagate. This therefore calls for the need to train the herbal practitioners on the appropriate propagation techniques of these plant species for sustainable utilization. According to Edwards [15], about two-thirds of 50, 000 medicinal plants in use worldwide are still harvested from the natural habitat and about one fifth of them are now endangered. The increased percentage of species obtained from the wild has a direct effect on the availability of these resources and is likely to contribute to their vulnerability to being overexploited.
The finding that majority of the informants interviewed were aged above 50 years augments Kisangau et al. [16]. This implies that the elderly people are the main custodians of traditional knowledge, and this poses a serious challenge of the knowledge gap between the elderly and the young generation if framework to ensure apprenticeship is not put in place.
Some herbal practitioners reported that there was a potential to domesticate medicinal plants as some of them were already being planted on farmlands. Kisangau et al. [17] support the observation that only a few herbal practitioners were involved in cultivation of medicinal plants and most of them were gathered from the wild. The unabated over collection of the medicinal plants from the wild is a major threat to their existence and raises serious concern for their conservation. In the present study, the few plant species that were found to be cultivated on farmlands included Senna petersiana (Bolle) Lock., Azadirachta indica A. Juss., Khaya anthotheca (Welw.) C. DC. and Moringa oleifera Lam. However, unavailability of planting material and lack of appropriate propagation techniques were noted to be the major constraints to exploiting the potential for medicinal plant domestication. On the other hand, species like Carica papaya L., Vernonia iodocalyx O. Hoffm., Helichrysum schimperi (Sch. Bip. ex A. Rich) Moeser and Citrus aurantifolia (Christm) Swingle. could easily be conserved by planting them in home gardens.
It was found that the most commonly harvested plant parts were leaves followed by roots. These results are contrary with the findings of Rukia [18] who reported that roots were the most commonly harvested plant parts followed by leaves in Udzungwa Mountains Forests in Tanzania.
Some methods of harvesting medicinal plants like root excavation and bark striping can be very devastating and a big threat to the plant survival. The high utilisation of roots has also been reported as putting many plant species at a risk of extinction because of the damages inflicted on them in the course of uprooting them [19,20]. Bark striping is also an equally harmful harvesting method as reported for Prunus africana and other medicinal plants in Cameroon [7]. In Namibia just like in other countries, harvesting of roots and barks was found to be common, destructive and unsustainable [21].
The fact that the most frequently utilised plant parts were leaves is a more sustainable practice as opposed to where roots and/or the bark are used. The prevalence in the use of leaves for preparation of traditional herbal remedies has been reported in other studies too [9,[22][23][24][25][26]. This practice helps to reduce the rate of threat on plant species and enhances the sustainable management of plants, as long as only an appreciable amount of leaves is harvested [27]. Leaves of plants have been reported to accumulate inulins, tannins and other alkaloids [28], which may be responsible for their various medicinal properties, hence explaining their wide use.

Conclusion
The results of the study revealed that there is rich diversity of medicinal plants used to treat various ailments in the neighbourhood of Kimboza forest reserve. Herbal practitioners and the local community in the study area should be educated on sustainable methods of harvesting medicinal plants without compromising their availability for future use. It is also imperative to train the community on the proper propagation techniques in order to encourage the domestication of valuable and threatened medicinal plants. The domestication of medicinal plants will create new opportunities for the local people such as provision of an alternative income and could help reduce the pressure on the wild population. Successful conservation strategies should be developed and priority given to sustainable harvesting of the plants.