Ethnobotanical assessment of plant resources of Banda Daud Shah, District Karak, Pakistan

Background The Indigenous knowledge of plants is scientifically and culturally very significant. This paper elucidates the empirical findings of an ethnobotanical survey of Banda Daud Shah, District Karak, Pakistan. Methods Data collection was carried out from October 2011 to September 2012. Total twelve survey trips were made, three in each season. About 100 respondents were interviewed; most of them were aged people between 60–70 years. Interviews were conducted using structured questionnaire composed of variety of questions regarding ethnomedicinal uses of plants of the study area. Direct matrix ranking (DMR), informant citations and market survey of multipurpose plants were also carried out. Results The local community was using 58 plant species belonging to 52 genera and 34 families for different purposes. A total of 25 plant species were herbs followed by 18 shrubs. Leaf (45%) was the most commonly used plant part followed by the whole plants (23%). In total, 40 plant species were medicinally used to treat variety of diseases, of which highest number of species being used for gastro-intestinal problems (19 spp.), expectorant (3 spp.) and antipyretic (3 spp.). Beside medicinal values, 25 species were used for fuel and 18 for fodder purposes. Informant consensus showed that gastrointestinal and respiratory infections were ranked highest (FIC = 0.75) among all ailments. According to DMR output, Dalbergia sisso ranked first due to high multipurpose uses among all species and was found most threatened with higher market value. Conclusion The investigated area is rural in nature and the inhabitants are highly dependent on the native plants for their health care needs and other requirements like fuel wood and fodder due to financial constraints and unavailability of resources. Medicinal plants for high ranked diseases may be phtyochemicaly and pharmacologically investigated to prove their efficacy. The local medicinal flora is facing overexploitation, overgrazing and improper way of collection. Proper conservation strategies such as controlled grazing, reforestation and rangeland management among many others may be adopted to promote the sustainable use of medicinal plants.


Introduction
Plants are a vital source of traditional medicines that are used for the treatment of various ailments [1]. Approximately 4, 22,000 flowering plants reported from the world, more than 50,000 have been used worldwide for medicinal purposes [2]. Main objective of ethnobotanical research is to record the indigenous uses of plant resources. Until now, 80% of the world's population depends on traditional medicines for its primary health care needs [3]. Plants remedies are often used as an alternative to allopathic medicines [4]. Inhabitants of the remote areas have good knowledge about the utilization of plants. Local people prefer medicinal plants due to their easy availability and cheap therapy as compared to costly pharmaceuticals. Local people have discovered the therapeutic activity of medicinal plants against certain diseases through their indigenous experiences transferred to them from their ancestors [5]. Recently, the field of ethnobotany has shown tendency from mere documentation process to a more practical one, emphasizing on the sustainable use of local medicinal flora. Treatment through traditional medicine system is progressing all over the world particularly in the Indian sub-contentment where this system is known by Unani or Ayurvedic [6].
Pakistan is bestowed with a unique biodiversity that is stretched along nine major ecological zones. A major part of country is quite rich in medicinal herbs due to its salubrious climate [7]. Numerous ethnomedicinal plants extracted from the wild are being used by local communities of different geographical regions having century's old traditional knowledge on such plants. A number of medicinal plants are well known about their properties and proper use at the community level, however many are yet to be explored for their medicinal values [8]. In early 1950 more than 80% of Pakistani population was totally dependent on ethnomedicines for traditional health practices [9], but now it is experienced only in the rural areas [10], because the indigenous knowledge develops and changes with the passage of time, with change of natural resources and culture. Indigenous knowledge on ethnomedicines is under threat due to the current modernizing trends among the rural societies, which is reducing the locals' wisdom on the precious flora. In Pakistan, about 6000 plant species have been reported so far, however ethnomedicinal knowledge on 600 plant species only have been documented [11]. There is a dire need to preserve this valuable traditional knowledge [12].
Preserving and promoting indigenous knowledge on medicinal plants are considered as rescuing the global heritage in addition to the new drugs discovery [13]. High priority should be given to the documentation of indigenous knowledge on the plants' ethnomedicinal use in order to ensure their protection for the present and future generation [14]. The present study was conducted in Banda Daud Shah, District Karak where agriculture is negligible and hence most people are dependent on the wild sources for different ethnobotanical purposes. Many studies have been conducted on the indigenous uses of medicinal plants in Pakistan [15][16][17][18][19][20][21][22][23] but investigated area is still unexplored. Therefore, a need was felt to document and conserve the traditional knowledge of the area before the information is lost forever. Hence, the present study aimed to explore the traditional knowledge, possible threats and conservation strategies with respect to the local flora. In addition, the study also aimed to select candidate medicinal plant species of high informants' consensus value. Specific objectives are (i) to identify medicinal plants and explore their uses as non timber forest products (NTFPs) (ii) to investigate the indigenous knowledge on ethnomedicines, and (iii) to assess the plants conservation issues of the study area.

Study area
Banda Daud Shah has a total area of 600 Km 2 and lies in the south of Khyber Pakhtunkhwa Province between 70-40°to 71-30°N latitude and 32-48°to 33-23°E longitude [14]. It is bounded in the North-West by District Hangu, in the North-East by District Kohat, in the South-West by Tribal area and in the South by Karak Tehsil (Figure 1). The study area is divided into mountainous area, small hills and plains having most of clayey or sandy soil. Wheat, corn and gram are the common cultivated crops. Various plant species dominated the study area such as Acacia modesta, Acacia nilotica, Adhatoda vasica, Aerva javanica, Dodonaea viscosa, Eucalyptus lanceolatus, Fagonia cretica, Rhazya stricta, Saccharum arundinaceum, Withania coagulans and Withania somnifera.

Informant selection
General information about the study area was collected before start of the research work. Medicinal plants grow in different seasons and therefore, ethnobotanical data was collected during summer, autumn, spring and winter from October 2011 to September 2012. Data was collected in twelve survey trips i.e. three in each season. Data collection and voucher specimens were collected following standard method of Martin [24]. Informants' sample size was selected on the basis of information provided by the local administrative officers. In total 100 native informants were selected as resource persons for data collection. Among all the informants, 70 were old age people (52 males, 18 females), which were aged between 50-70 years. Whereas, ages of 19 informants ranged between 30-45 years, in which mostly females (13) were interviewed. Remaining 11 informants (6 males, 5 females) were below 30 years of age. The selected respondents were experts in field of medicinal plants and were having great reputation in the society about their knowledge on traditional medicines. These informants were social workers, farmers, rural herbalists (Hakeem's) and housewives. Before data collection, a brief group discussion was held with the key informants, in which objectives of research were explained to them. This was done in order to acknowledge informants' cooperation in preserving the traditional knowledge of the study area and build their confidence for providing reliable information.

Data collection, organization and analysis
Structured questionnaire was designed containing predefined questions about medicinal plants. Informants were asked about the local names of plants, ailment treated, part used, other uses beside medicinal, most commonly used plants. In addition, focus group discussions were also designed so as to gain further information on medicinal plants knowledge of the community and prove the reliability of the data collected through questionnaire. Interviews were conducted in the local language (Pashto) by visiting each respondent individually. The habits of the studied plants were documented from the online literature [25]. Plant parts were classified into 8 categories like leaves, roots, whole plant etc. Other uses besides medicinal uses were grouped into 11 categories like fuel wood, sheltering plants, fodder, agricultural tools etc. Habits of the plants were categorized into three parts like herbs, shrubs and trees.
Data on use diversity of multipurpose medicinal plants were evaluated by a direct matrix ranking (DMR) exercises as described in Cotton [26] that involved fifteen (ten men and five women) key informants. Participants for this exercise were selected based on their long years of experience as traditional herbal practitioners in the study area as described in Yineger et al. [27]. Market survey was also conducted for multipurpose medicinal plants. Market value of medicinal plants was recorded as US$ Kg -1 for prices uniformity.
Informant consensus factor (F IC ) was computed after the reported traditional remedies and corresponding diseases were grouped into 12 categories. F IC was obtained by computing number of use citations in each disease category (Nur) minus the number of times a species used (Nt), divided by the number of use citations in each category minus one [28].

Preservation of plants
The plants were pressed, dried and mounted on the herbarium sheets. Plants were identified by the expert taxonomists at the Department of Botany, Kohat University of Science and Technology. Scientific names (Latin), family names and names of publication authors were corrected according to the Flora of Pakistan [25]. For confirmation, the studied plants samples were compared with the already identified plant specimens preserved in the herbarium of University of Peshawar, Pakistan. The correctly identified specimens were deposited in the herbarium, Department of Botany, Kohat University of Science and Technology, Kohat.

Results
A total of 58 plant species belonging to 52 genera and 34 families were collected from the study area. All the species were identified and their ethnobotanical information was elucidated ( Table 1). Out of 34 families, 3 were monocotyledons (Arecaceae, Poaceae and Typhaceae) and the remaining 31 families belonged to dicotyledons. The dominant family was Solanaceae (5 spp.), followed by Asclepiadaceae, Asteraceae, Brassicaceae, Mimosaceae, Papalionaceae and Poaceae each were sharing 3 species (Table 1). Out of total plant species, 25 were herbs followed by 18 shrubs species (Figure 2). Moreover, out of the total 58 plant species, 40 were used for the preparation of various ethnomedicines, 25 as fuel wood and 18 were used as fodder species (Figure 3). Mostly the leaves of 45% plant species were used for medicinal purpose followed by 23% species used as a whole plant ( Figure 4). Out of the 40 medicinal plants, 19 were used against gastro-intestinal disorders followed by 3 each as expectorant and antipyretic ( Figure 5). The output of the DMR exercise on ten multipurpose medicinal plants enabled to identify which of the multipurpose plants is most under pressure in the area and the corresponding factors that threaten the plant. Accordingly, Dalbergia sisso ranked first (most threatened); Acacia modesta and Acacia nilotica ranked second; Morus nigra ranked third; Ziziphus nummularia ranked fourth, while Monotheca buxifolia and Capparis desidua ranked fifth ( Table 2). Results also indicated that those multipurpose medicinal plant species are currently exploited more for construction, firewood and fodder purposes than for their medicinal role. Market survey showed that among tree species, Dalbergia sissoo ranked first with higher market price (0.6 US$ Kg -1 ) ( Table 2). Table 3 showed the informant consensus factor (F IC ) for different ailments treated by traditional healers in the study area. The level of informants agreement was high for most ailment categories (mean F IC = 0.75) like gastrointestinal and respiratory infections and total consensus (F IC = 1.00) was even obtained for dermatological, inflammations, cardiovascular, fever and dental problems. Results also showed that no ailments have low level of consensus.

Discussion
The study reveals that people of the area are much dependant on the native flora for acquiring their basic requirements such as medicines, fodder, fruits, vegetables, fuel, furniture and roof thatching. The local people utilize over 58 plants in their day to day life. All these species are the main sources of traditional health care needs and economic opportunity for the local people. Traditional healers of the study area mostly use herbs in the remedy preparation for the treatment of various ailments, which might be due to their strong therapeutic activity and easy extraction from the wild. Our study is in line with the findings of Mohammed [29], Gebre [30] and Teklehaymanot and Giday [31], who have enlisted various ethnomedicinal recipes being prepared from herb species. Family Solanaceae is the most dominant family in the study area, which mostly includes herbs and shrubs, play a vital economic role in the present study. The family is being used either as fuel wood or as medicinal species and are thus highly preferred.
The traditional healers and local herbalists of the region usually make use of every part of the plant. However, the use of a particular plant part depends on the plant habit and user's needs. The most frequently used plant parts in the preparation of herbal remedies were leaves and whole plant. The use of specific plant parts suggests that these parts have strongest medicinal properties but it needs biochemical analysis and pharmaceutical screening to cross-check the local information. Our findings of the frequent use of green leaves in the preparation of remedies corroborate the results of [32][33][34].
Harvesting of whole plant for remedy preparation causes great danger to the plant population.
Main ailments in the study area are related to gastrointestinal problems, respiratory infections and fever. The possible reason behind such ailments in the investigated region might be due to high water and air pollution, lack of proper sanitation, fuel wood smoke inside houses and poor quality food. Gastro-intestinal disorders and respiratory infections, particularly cholera, diarrhea and dysentery, cough, asthma and bronchitis are a major concern not only in the study area but the whole country and result in high mortality rate if not treated promptly [35]. These findings are similar to an ethnobotanical study conducted in the other parts of Pakistan, where species are locally used for related medicinal purposes [12,36]. Total seventeen species such as Calotropis procera, Convolvulus arvensis, Capparis deciduas and Acacia nilotica were found to be effective ethnomedicines for the treatment of gastrointestinal disorders. Murad et al. [37] also described the same plants using for the same ailment in Malakand region of Pakistan. Majority of the plants among them are used for the treatment of more than one type of ailment. This cultural similarity and multiple medicinal uses of single plant are the strong indication of availability of variety of therapeutic phytochemical. The highest recorded F IC values (1.00, 0.88 and 0.71) indicated best agreement among informants' on the use of medicinal plant species reported to be used for treating gastrointestinal, respiratory, fever, cardiovascular diseases respectively. The observed highest informants' agreement coupled with high plant use citations for disease categories like gastrointestinal and respiratory infections could also indicate the relatively high incidence of the latter diseases in the area. According to Heinrich et al. [28], high F IC values are important to identify plants of particular interest in the search for bioactive compounds. Plants use for disease categories  such as fever, cardiovascular, dental and inflammatory infections recorded highest F IC value and could be search for their bioactive compounds, however in comparison to gastrointestinal and respiratory infections these categories observed small number of plant use citation and less number of taxa. Tolossa et al. [38] also found gastrointestinal infection as one of the common ailment of the Ethiopian region. The output of a DMR showed highest values (ranks) for a number of multipurpose medicinal plants of the study area such as Dalbergia sisso, Acacia modesta, Acacia nilotica, Capparis desidua, Ziziphus nummularia and Monotheca buxifolia. The result indicates that these plants are exploited more for their non-medicinal uses than for reported medicinal values. Overharvesting of multipurpose medicinal plant species for construction, fuel wood, fodder agricultural tools and other purposes were found the responsible factors aggravating depletion of the species in the area. Thus, the result calls for an urgent complementary conservation action to save the fast eroding multipurpose medicinal plant species of the area. Same pattern of highest exploitation of multipurpose medicinal plants for uses other than their traditional importance has been found [39]. Dalbergia sisso is the most valued species in the study area and DMR. According to Khan and Khan [40] the wood of Dalbergia sisso is highly preferred fuelwood and timber species. The informants selected for direct matrix ranking also indicated that most of the people of the region are also involved in exporting timber of Dalbergia sisso to the industries located in other regions of Pakistan for generating their income apart from their doemestic use as furniture and fuel wood. A study conducted in Gujrat region Pakistan indicated that eighty percent of industrial furniture is being made from Dalbergia sisso [41]. Other species like Acacia nilotica, Acacia modesta, Ziziphus nummularia, Capparis desidua and Monotheca buxifolia are also the most frequently using species for other non timber forest products. Zaman and Khan [42], Shahid ullah [43] and Ahmad [44] also reported   multipurpose uses of the aforementioned species. Our findings are also in line with the study by Barkat et al. [45] carried out in district Malakand. He found that in the absence of gas supply and other fuel types in the area, the local people extensively use tree species as fuel wood. Ejaz et al. [46] also recorded the similar results in their study conducted in new Muree, Pakistan. Species such as Acacia modesta, Asphodelus tenuifolius, Capparis desidua, Peganum hermala, Zizyphus jujuba are considered as wild honeybee attraction species [47]. Honey of Zizyphus species is the most famous among the locals of study area due to its taste and medicinal value. Fine quality of honey is exported to markets at the provincial and national levels. Overharvesting and increasing trade of medicinal plants has significantly reduced the availability of the medicinal plants in arid and semi-arid region [48]. The present study observed that a large numbers of plants like are over harvested for medicinal and other NTFPs uses. Agriculture and livestock raring in the study area are common activities also to support rural livelihood. Therefore grazing is posing another pressure on the flora of the region. As an example, animals' trampling can make the soil compact that retard seed germination and seedlings growth [49]. Moreover, goats raring are common in the study area, which can cause serious damage to plants through browsing. It is thus imperative to take necessary steps to protect the forests from such practices and device plans for range land management in order to improve the status of local vegetation. It was noted that ethnomedicinal knowledge is becoming restricted only to the elders, Hakeem's (traditional practitioners) and Pensaries (local herb sellers); while young people are totally ignorant of this wealth. Advancement in science and technology has changed the social values and therefore, younger generation are transforming at a much faster rate into the new tradition. Medicinal plants knowledge is going to be obsolete because of the interference of modern cultural changes. It is therefore very important to document the native flora along with their ethnomedicinal recipes before extinction of the indigenous knowledge.

Conclusion
It is concluded from the present study that the natives of the region are very much dependent on plant species for their health care needs, fuel wood and fodder. Due to financial constraints, changing life styles and unavailability of resources, the local medicinal flora is facing overexploitation from the local inhabitants. Over grazing, fodder collection, logging and medicinal plants collection are major threats to the vegetation of the studied area. Such practices have resulted in the vulnerability of multipurpose species such as Acacia modesta and Dalbergia sisso. Hence, certain precautionary measures (controlled grazing, reforestation, rangeland management etc.) need to be addressed for the protection of threatened species. Moreover, in-situ and ex-situ conservation methods should be practiced as long-term conservation programme. Conservation education may be extended to the local communities and their local technologies may be incorporated in developing plans. Community mobilization and involvement may be assured in natural conservation. Alternative resources should be  Construction 5  2  3  1  2  3  4  3  1  1  25  2   Agriculture  4  2  2  3  2  2  2  1  1  1  20  5   Firewood  3  4  3  2  3  2  3  2  2  2  26  1   Fodder  2  3  3  2  2  3  3  2  3  1  24  3   Medicinal  1  3  3  3  2  2  1  2  3  3  23  4   Total  15  14  14  11  11  12  13  10   explored to reduce indiscriminate use and cutting for fuel purposes. Horticulture crops especially fruits, off season vegetable and mushrooms culture may be extended for the economic uplift of the area and reducing pressure of fuel wood on the forests. Moreover, there is an urgent need to document the traditional knowledge of the area, which is another step towards the conservation of local flora. The local community may be educated and trained for the collection and sustainable use of medicinal flora. Additionally, the native healers may also be encouraged to accurately inculcate their traditional knowledge to local community.