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Table 1 Gene regions, primers and amplification protocols used for polymerase chain reaction

From: Quantitative ethnopharmacological documentation and molecular confirmation of medicinal plants used by the Manobo tribe of Agusan del Sur, Philippines

Gene region Primer name Reference Primer sequence (5′ ➔ 3′) PCR Protocol
ITS (ITS1, 5.8S gene, and ITS2) p17F [57] 5′-CTACCGATTGAATGGTCCGGTGAA-3′ 94 °C 5 min; 28 cycles of 94 °C 1 min, 48 °C 1 min, 72 °C 1 min; 72 °C 7 min; 10 °C paused
26S-82R 5′-TCCCGGTTCGCTCGCCGTTACTA-3′
5 [58] 5′-GGAAGTAAAAGTCGTAACAAGG-3′ 94 °C 5 min; 30 cycles of 94 °C 1 min, 55 °C 1 min, 72 °C 1 min, 45 s; 72 °C 10 min; 10 °C paused
4 5′-TCCTCCGCTTATTGATATGC-3′
matK 3F_kimF [59] 5′-CGTACAGTACTTTTGTGTTTACGAG-3′ 98 °C 45 s; 35 cycles of 98 °C 10 s, 52 °C 30 s, 72 °C 40 s; 72 °C 10 min; 10 °C paused
IR_kimR 5′-ACCCAGTCCATCTGGAAATCTTGGTTC-3′
psbA-trnH psbA_F [60] 5′-GTTATGCATGAACGTAATGCTC-3′ 95 °C 4 min; 35 cycles of 94 °C 30 s, 55 °C 1 min, 72 °C 1 min; 72 °C 10 min; 10 °C paused
trnH_R 5′-CGCGCATGGTGGATTCACAATCC-3′
trnL-F c [61] 5′-CGAAATCGGTAGACGCTACG-3′ 94 °C 3 min; 30 cycles of 93 °C 1 min; 55 °C 1 min, 72 °C 2 min; 10 °C paused
f 5′-ATTTGAACTGGTGACACGAG-3′