Table 1 Gene regions, primers and amplification protocols used for polymerase chain reaction

ITS (ITS1, 5.8S gene, and ITS2)

p17F

[57]

5′-CTACCGATTGAATGGTCCGGTGAA-3′

94 °C 5 min; 28 cycles of 94 °C 1 min, 48 °C 1 min, 72 °C 1 min; 72 °C 7 min; 10 °C paused

26S-82R

5′-TCCCGGTTCGCTCGCCGTTACTA-3′

5

[58]

5′-GGAAGTAAAAGTCGTAACAAGG-3′

94 °C 5 min; 30 cycles of 94 °C 1 min, 55 °C 1 min, 72 °C 1 min, 45 s; 72 °C 10 min; 10 °C paused

4

5′-TCCTCCGCTTATTGATATGC-3′

matK

3F_kimF

[59]

5′-CGTACAGTACTTTTGTGTTTACGAG-3′

98 °C 45 s; 35 cycles of 98 °C 10 s, 52 °C 30 s, 72 °C 40 s; 72 °C 10 min; 10 °C paused

IR_kimR

5′-ACCCAGTCCATCTGGAAATCTTGGTTC-3′

psbA-trnH

psbA_F

[60]

5′-GTTATGCATGAACGTAATGCTC-3′

95 °C 4 min; 35 cycles of 94 °C 30 s, 55 °C 1 min, 72 °C 1 min; 72 °C 10 min; 10 °C paused

trnH_R

5′-CGCGCATGGTGGATTCACAATCC-3′

trnL-F

c

[61]

5′-CGAAATCGGTAGACGCTACG-3′

94 °C 3 min; 30 cycles of 93 °C 1 min; 55 °C 1 min, 72 °C 2 min; 10 °C paused

f

5′-ATTTGAACTGGTGACACGAG-3′