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Table 1 Gene regions, primers and amplification protocols used for polymerase chain reaction

From: Quantitative ethnopharmacological documentation and molecular confirmation of medicinal plants used by the Manobo tribe of Agusan del Sur, Philippines

Gene regionPrimer nameReferencePrimer sequence (5′ ➔ 3′)PCR Protocol
ITS (ITS1, 5.8S gene, and ITS2)p17F[57]5′-CTACCGATTGAATGGTCCGGTGAA-3′94 °C 5 min; 28 cycles of 94 °C 1 min, 48 °C 1 min, 72 °C 1 min; 72 °C 7 min; 10 °C paused
26S-82R5′-TCCCGGTTCGCTCGCCGTTACTA-3′
5[58]5′-GGAAGTAAAAGTCGTAACAAGG-3′94 °C 5 min; 30 cycles of 94 °C 1 min, 55 °C 1 min, 72 °C 1 min, 45 s; 72 °C 10 min; 10 °C paused
45′-TCCTCCGCTTATTGATATGC-3′
matK3F_kimF[59]5′-CGTACAGTACTTTTGTGTTTACGAG-3′98 °C 45 s; 35 cycles of 98 °C 10 s, 52 °C 30 s, 72 °C 40 s; 72 °C 10 min; 10 °C paused
IR_kimR5′-ACCCAGTCCATCTGGAAATCTTGGTTC-3′
psbA-trnHpsbA_F[60]5′-GTTATGCATGAACGTAATGCTC-3′95 °C 4 min; 35 cycles of 94 °C 30 s, 55 °C 1 min, 72 °C 1 min; 72 °C 10 min; 10 °C paused
trnH_R5′-CGCGCATGGTGGATTCACAATCC-3′
trnL-Fc[61]5′-CGAAATCGGTAGACGCTACG-3′94 °C 3 min; 30 cycles of 93 °C 1 min; 55 °C 1 min, 72 °C 2 min; 10 °C paused
f5′-ATTTGAACTGGTGACACGAG-3′