Ethnobotany of wild plants used for starting fermented beverages in Shui communities of southwest China
© Hong et al.; licensee BioMed Central. 2015
Received: 7 January 2015
Accepted: 1 May 2015
Published: 28 May 2015
Shui communities of southwest China have an extensive history of using wild plants as starters (Xiaoqu) to prepare fermented beverages that serve important roles in interpersonal relationships and cultural events. While the practice of using wild plants as starters for the preparation of fermented beverages was once prevalent throughout China, this tradition has seen a decline nationally since the 1930s. The traditional technique of preparing fermented beverages from wild plant starters remains well preserved in the Shui communities in southwest China and provides insight on local human-environment interactions and conservation of plant biodiversity for cultural purposes. The present study sought to examine the ethnobotany of wild plants used as starters for the preparation of fermented beverages including an inventory of plants used as a starter in liquor fermentation and associated knowledge and practices.
Field surveys were carried out that consisted of semi-structured surveys and plant species inventories. One hundred forty-nine informants in twenty Shui villages were interviewed between July 2012 and October 2014 to document knowledge associated with wild plants used as a liquor fermentation starter. The inventories involved plant voucher specimens and taxonomic identification of plant collections.
A total of 103 species in 57 botanical families of wild plants were inventoried and documented that are traditionally used as starters for preparing fermented beverages by Shui communities. The majority of the species (93.2%) have multiple uses in addition to being used as a starter with medicinal purposes being the most prevalent. Shui women are the major harvesters and users of wild plants used as starters for preparing fermented beverages and transfer knowledge orally from mother to daughter.
Findings from this study can serve as a basis for future investigation on fermented beverages and foods and associated knowledge and cultural practices. However, with rapid development, utilization of wild plants and the cultural systems that support them are at risk of erosion. Cultural preservation practices are necessary in Shui communities for the continued use and transmission of this ethnobiological knowledge as well as associated biodiversity.
Fermented food and beverages that preserve diverse, locally available resources have been consumed for centuries worldwide as notable dietary components to support household food security and overall wellbeing [1–10]. Traditionally, such products were associated with cultural identity and social aspects of communities and were most often prepared at the household-scale through the action of microorganisms and their enzymes [10,11]. Key characteristics of fermented foods and beverages are enhancements to flavor and/or appearance, preserved quality, prolonged shelf-life, reduced cooking time and prebiotic and probiotic properties that have benefits for increasing digestability and bioavailablilty of certain nutrients [12–19]. Various cultures around the world prepare and consume fermented products to enhance their basic diet including as a side dish, condiment, pickle, confection and beverage. Knowledge on the preparation and attributes of fermented foods and beverages has been transferred from generation to generation and represents traditional ethnobiological knowledge.
In many minority socio-linguistic groups throughout China, the preparation and consumption of fermented alcoholic beverages are important cultural practices that define many social interactions including rituals during courtship, engagements, weddings, hospitality, funerals, ancestor worship and other ceremonies . Various socio-linguistic groups have their own characteristics of preparing and consuming fermented alcoholic beverages that contribute to cultural identity such as Mongolian koumiss, Yi’s spicy liquor, Tibetan highland barley wine and Shui’s Jiuqianjiu liquor.
The Shui prepare fermented alcoholic beverages known in Chinese as Jiuqianjiu liquor and in Shui as Kaojiuqian that is made from water, rice and a special starter made of wild plants known as Xiaoqu. Although the origin is not clear, Jiuqianjiu liquor is a traditional fermented beverage that has long been prepared and consumed in Shui communities as an integral part of daily life as well as for celebratory reasons including the main Shui holiday that occurs in the lunar calendar during September. The production of Jiuqianjiu liquor involves harvesting wild plants and the wild type microbe inoculation of molds, yeasts and bacteria in a rice (or other grain) substrate. The whole process of making this starter from plant collecting to material mixing, shaping, ripening, drying and storing usually takes 3 months. The Xiaoqu starter is often regarded as the most important ingredient for determining the quality of the final beverage. The production of Jiuqianjiu liquor can be compared to the Japanese process of Koji. Diverse wild plants are used as a starter for preparing fermented alcoholic beverages in Shui and other socio-linguistic groups of southwestern China. Indigenous communities in southwestern China believe that the synergy of different wild plants with microorganisms modifies the environment for the microbes by providing nutrition and inhibiting the growth of detrimental microbes and ultimately can modify the flavor and health attributes of the final fermented product.
The use of wild plants as a starter for fermented beverages has a long history in China. The earliest known recording is by Qi Min Yao Shu during the years 533–544. With technological development, pure breeding fermentation technologies that were introduced to China in the 1930s replaced the use of wild plants as a fermentation starter in most areas throughout the country. In the present era, socio-economic and political influences in China’s rural areas are threatening traditional practices of preparing fermented foods and beverages along with their associated knowledge base.
Understanding the ethnobotany of wild plants used as a starter for preparing fermented beverages can serve as a basis for future studies and applications regarding fermented products. However, this information has not been documented in most communities where this practice remains prevalent. The present study tries to address this knowledge gap through an ethnobotanical investigation of wild plants used as starters for fermented beverages in indigenous Shui communities of southwest China. Specifically, our study aimed to address the following objectives in Shui communities: (1) characterize the plants used as starters to prepare fermented alcoholic beverages, (2) document associated ethnobotanical knowledge, and (3) record the processing of fermented alcoholic beverages.
Materials and methods
Study area and Shui people
Surveys were conducted in Sandu Shui Autonomous County located in the south of Guizhou Province of China (25°30′-25°10′ N, 107°40′-108°14′ E). It is in Qiannan Prefecture, Guizhou province where the Shui population is most densely settled and where the Shui people regard it as their cultural and linguistic center . This area was selected for research because it is floristically rich with a cultural practice of drawing on this biodiversity for preparation of fermented beverages. Sandu Shui Autonomous County is characterized by numerous high mountains and large and small rivers. The climate is considered as a subtropical humid monsoon type with long summers and short winters. The complexity of the terrain, topography and altitude of the county has resulted in enormous variations of the climate. The country has a total area of 2,380 square kilometers with a total of 237,588 people living in 270 villages.
With an approximate population of 406,900, the Shui are one of the 55 officially recognized minority nationalities in China. Sandu Shui Autonomous County is the only county in China dominated by Shui people. The Shui people account for 65.93% of the total population of this prefecture; the remaining population consists of Han, Buyi, Miao, Yao and nine other minority socio-linguistic groups. They are distributed in Guizhou, Guangxi, Yunnan and Sichuan Provinces. The Shui language belongs to the Kam-Shui language grouping within the Sino-Tibetan language family [21, 22]. Due to the long communication with the Han, almost all Shui now know both Shui and Chinese Mandarin languages. While the Shui people have their own written language as found in the “Shui Book”, Chinese has become the written language in their daily life. The Shui also have their own calendar that dictates their ceremonies.
These Shui villages in mountainous valleys and basins are usually located near rivers and even today display the stilted wooden house style. The Shui at the study sites live in clusters of small-scaled villages. Until some decades ago there was a custom that a Shui woman should get married to one of her father’s sister’s son (cross-cousin marriage). Some have suggested that cross-cousin bonds reflected an original clan-based social organization with compact communities and villages typically of a few hundred related by bloodlines [21, 23]. Historically, they were not allowed to intermarry with other nationalities. Similar to other indigenous groups in the area, the Shui follow polytheism and animism with worship of ancestors and natural objects including mountains, rocks and ancient trees. Traditional lifestyle is still common in the area and the Shui people are fond of pickles and sour soup in their daily diet. Staple food of the Shuis is rice, together with different local vegetables and meat as protein source. Traditional practices are still common in Shui communities including the production of fermented alcoholic beverages.
Plants used for liquor fermentation starter in Sandu County of Guizhou
Achyranthes aspera L.
Clearing away heat and toxic materials; Diuretics; Cold; Fever; Amygdalitis; Mumps
Actinidia chinensis Planch.
The fruit is edible
Actinidia rubricaulis var. coriacea (Finet & Gagnep.) C.F. Liang
The fruit is edible
Agrimonia pilosa Ledeb.
Ainsliaea fragrans Champ. ex Benth.
Heat-clearing and detoxifying effect; Clearing away heat and toxic materials; Diuretics; Hematemesis; Traumatic injury
Akebia trifoliata (Thunb.) Koidz.
Diuretics; Promoting lactation; Loosing bones and muscles; Removing dampness; Arthralgia
Anaphalis bicolor (Franch.) Diels
Removing dampness; Relieving summer-heat; Cough
Antenoron filiforme (Thunb.) Roberty & Vautier
Dispelling wind and cold; Removing dampness; Relieving pain; Stopping bleed; Eliminating stasis to stop pain
Ardisia japonica (Thunb.) Bl.
Phthisis; Hemoptysis; Cough; Tracheitis; Removing dampness; Leukorrhea; Amenorrhoea; Diuretics
Artemisia anomala S. Moore
Removing dampness; Promoting blood circulations; Eliminating stasis to stop pain; Irregular menstruation; Relieving pain
Asarum splendens (F. Maekawa) C. Y. Cheng & C. S. Yang
Dispelling wind and cold; Relieving pain; Warming lung for dispelling cold
Asarum wulingense C.F. Liang
Dispelling wind and cold; Relieving pain; Warming lung for dispelling cold
Asplenium trichomanes L.
Heat-clearing and detoxifying effect; Removing dampness; Stopping bleed; Eliminating stasis to stop pain; Dysentery; Leukorrhea; Irregular menstruation; Backache
Aster ageratoides Turcz.
Cold; Cough; Asthma; Consumptive disease; Blood vomitting
Aster tataricus L. f.
Cold; Cough; Asthma; Consumptive disease; Blood vomitting
Betula insignis Franch.
Cough; Asthma; Eliminating phlegm
Bletilla striata (Thunb.) Rchb. f.
Stopping bleed; Anti-swelling agent; Promoting tissue regeneration
Brandisia hancei Hook. f.
Clearing away heat and toxic materials; Bone fracture; Hepatitis
Campanumoea javanica Bl.
Warming spleen and stomach for dispelling cold; Moistening lung to arrest cough; Inducing saliva and slakes thirst; Spleen tonic
Carpesium cernuum L.
Clearing away heat and toxic materials; Anti-swelling agent; Anti-inflammation; Relieving pain; Cold; Fever; Toothache; Dysentery; Diuretics
Ceropegia trichantha Hemsl.
Clearing away heat and toxic materials; Bone fracture; Hepatitis; Ornamental
Cinnamomum cassia (L.) D. Don
Relieving pain; Waist and knee pain; Stomachache; Dyspepsia; Amenorrhea; Spice
Citrus reticulata Blanco
Regulating qi-flowing for eliminating phlegm; Promoting blood circulations; Anti-swelling agent; The fruit is edible
Codonopsis pilosula (Franch.) Nannf.
Invigorating the blood; Spleen and stomach tonic; Weakness; The root is cooking for edible
Commelina bengalensis L.
Clearing away heat and toxic materials; Diuretics; Anti-swelling agent; The leaves and stems are cooking as vegetables
Cornus hongkongensis Hemsl.
Ornamental, The fruit is edible
Cunninghamia lanceolata (Lamb.) Hook.
Cyclobalanopsis glauca (Thunb.) Oerst.
Clearing away heat and toxic materials
Dichrocephala integrifolia (L. f.) Kuntze
Clearing away heat and toxic materials; Rremoving dampness
Diospyros kaki var. silvestris Makino
The fruit is edible
Elaeagnus pungens Thunb.
Dispelling wind and cold; Removing dampness; Removing blood stasis; Stopping bleed; Traumatic injury
Elsholtzia ciliata (Thunb.) Hyl.
Relieving exterior and sweating; Removing dampness; Warming spleen and stomach for dispelling cold; Diuretics; Anti-swelling agent
Ficus henryi Warb.
Cough; Toothache; Removing dampness; The fruit is edible
Ficus pumila L.
Clearing away heat and toxic materials; Expelling damp; Diuretics; The achene is washed for cooking bean jelly
Ficus tikoua Bureau
Stem, Leaf, Fruit
Heat-clearing and detoxifying effect; Removing dampness; Promoting blood circulations; Clearing away heat and toxic materials; The fruit is edible
Flemingia prostrata Roxb.
Dispelling wind and cold; Removing dampness; Removing blood stasis; Clearing away heat and toxic materials
Gardenia jasminoides J. Ellis
Heat-clearing and detoxifying effect; Purging intense heat; Herbal tea; Ornamental
Gaultheria griffithiana Wight
Removing dampness; Ornamental
Gaultheria leucocarpa Bl.
Removing dampness; Ornamental
Gerbera piloselloides (L.) Cass.
Clearing internal heat; Anti-inflammatory effects; Cold; Fever; Postpartum dysphoria
Gerbera anandria (L.) Sch.-Bip.
Heat-clearing and detoxifying effect; Removing dampness; Anti-swelling agent; Bleeding
Geum aleppicum Jacq.
Irregular menstruation; Dispelling wind and cold; Removing dampness; Relieving pain
Glochidion puberum (L.) Hutch.
Stem, Leaf, Fruit
Heat-clearing and detoxifying effect; Removing dampness; Dispelling wind and cold; Loosing bones and muscles; Leukorrhea; Dysmenorrhea
Glycosmis pentaphylla (Retz.) DC.
Eliminating stasis to stop pain; Anti-swelling agent; The fruit is edible
Gonostegia hirta (Bl. ex Hassk.) Miq.
Spleen tonic; Digestion; Heat-clearing and detoxifying effect; Removing dampness; Anti-swelling agent
Haloragis micrantha Thunb.
Diuretics; Expelling damp; Clearing away heat and toxic materials; Antidysmenorrhea; Promoting blood circulations
Hedera nepalensis K. Koch
Dispelling wind and cold; Removing dampness; Promoting blood circulations; Anti-swelling agent; Ornamental
Hedyotis auricularia L.
Heat-clearing and detoxifying effect; Removing dampness; Anti-swelling agent
Hovenia acerba Lindl.
Promoting blood circulations; Eliminating stasis to stop pain; Clearing heat and expelling damp; Asthma; Ornamental; Fence
Hylodesmum podocarpum subsp. fallax (Schindl.) H. Ohashi & R.R. Mill
Dispelling wind and cold; Loosing bones and muscles; Clearing away heat and toxic materials; Anti-swelling agent; Traumatic injury; Removing dampness; Backache
Hylodesmum podocarpum subsp. oxyphyllum (DC.) H. Ohashi & R.R. Mill
Dispelling wind and cold; Loosing bones and muscles; Clearing away heat and toxic materials; Anti-swelling agent; Traumatic injury; Removing dampness
Hypericum japonicum Thunb.
Heat-clearing and detoxifying effect; Removing dampness; Promoting blood circulations; Anti-swelling agent
Hypericum sampsonii Hance
Clearing away heat and toxic materials; Restoring menstrual flow; Loosing bones and muscles; Stopping bleed; Fever; Dysentery; Irregular menstruation,Leukorrhea
Imperata cylindrica (L.) P. Beauv.
Stopping bleed; Heat-clearing and detoxifying effect; Diuretics
Indigofera zollingeriana Miq.
Young stem, Leaf
Clearing away heat and toxic materials; Removing blood stasis; Stopping bleed
Inula cappa (Buch.-Ham. ex D. Don) DC.
Dispelling wind and cold; Anti-swelling agent; Relieving pain
Iris tectorum Maxim.
Promoting blood circulations; Removing blood stasis; Dispelling wind and cold; Removing dampness; Clearing away heat and toxic materials; Digestion; Ornamental
Kadsura longipedunculata Finet & Gagnep.
Astringent; Inducing saliva and slakes thirst; Notifying kidney and spleen; The fruit is edible
Leontopodium leontopodioides (Willd. ) Beauv.
Clearing away heat and toxic materials
Leonurus japonicus Houtt.
Young stem, Leaf
Promoting blood circulations; Removing blood stasis; Diuretics; Anti-dysmenorrhea
Lilium brownii F.E. Brown ex Miell.
Moistening lung to arrest cough; Heat-clearing and detoxifying effect; Tranquilizing the mind; Diuretics; The bulb is edible
Litsea cubeba (Lour.) Pers.
Warming spleen and stomach for dispelling cold; Dispelling wind and cold; Anti-swelling agent; Spice
Lonicera lanceolata Wall.
Stem, Leaf, flower
Lophatherum gracile Brongn.
Reducing pathogenic fire; Fever; Diuretics; Herbal tea
Lygodium japonicum (Thunb.) Sw.
Clearing away heat and toxic materials; Removing dampness; Relieving pain Urinary tract infections; Hepatitis; Nephritis edema; Diarrhea
Mahonia bodinieri Gagnep.
Clearing away heat and toxic materials; Anti-swelling agent; Antidiarrheic; Dysentery; Hepatitis; Ornamental
Melastoma dodecandrum Lour.
Removing toxicity; The fruit is edible
Mosla scabra (Thunb.) C.Y. Wu & H.W. Li
Cold; Malaria; Constipation; Hemafecia; Bleeding; Traumatic injury
Myrica rubra (Lour.) Sieb. & Zucc.
Inducing saliva and slakes thirst; Digestion; The fruit is edible
Nephrolepis biserrata (Sw.) Schott
Cold; Fever; Cough; Tuberculosis; Dysentery; Enteritis; The tuber is edible
Ohwia caudata (Thunb.) H. Ohashi
Onychium japonicum (Thunb.) Kze.
Clearing away heat and toxic materials; Removing dampness; Stopping bleed; The leaves are cooked as vegetables
Paederia foetida L.
Dispelling wind and cold; Removing dampness; Digestion; Clearing away heat and toxic materials; Anti-swelling agent; Promoting blood circulations; Relieving pain
Phyllagathis cavaleriei (Levl. et Van.) Guillaum.
Reducing pathogenic fire; Tonic
Phyllagathis longiradiosa C. Chen
Clearing away heat and toxic materials
Piper hancei Maxim.
Dispelling wind and cold; Removing dampness; Activating collaterals
Plantago depressa Willd.
Clearing away heat and toxic materials; Diuretics; The whole plants is cooked as vegetables
Platycodon grandiflorus (Jacq.) A. DC.
Cough;Eliminating phlegm; Ornamental
Polygala japonica Houtt.
Promoting blood circulations; Eliminating stasis to stop pain; Eliminating phlegm and relieve cough; Clearing away heat and toxic materials; Relieving pain
Polygonum hydropiper L.
Clearing away heat and toxic materials; Eliminating stasis to stop pain;stopping bleed; Dysentery; Traumatic injury; Spice
Portulaca oleracea L.
Clearing away heat and toxic materials; Promoting circulation and removing stasis; Anti-swelling agent; The aerial part is cooked as vegetabl
Pothos chinensis (Raf.) Merr.
Dispelling wind and cold; Removing dampness; Promoting blood circulations; Eliminating stasis to stop pain; Digestion; Cough
Pueraria montana (Lour.) Merr.
Fever; Inducing saliva and slakes thirst; Inducing saliva and slakes thirst; Antidiarrheic; Kudzu powder is edible
Rohdea japonica (Thunb.) Roth
Clearing away heat and toxic materials; Eliminating stasis to stop pain; Relieving pain
Rosa roxburghii Tratt.
Heat-clearing and detoxifying effect; Inducing saliva and slakes thirst; Digestion; The fruit is edible
Rosa laevigata Michx.
Promoting blood circulations; Eliminating stasis to stop pain; Dispelling wind and cold; Removing dampness; Clearing away heat and toxic; The fruit is ediblematerials
Rubus alceaefolius Poir.
Promoting blood circulations; Removing blood stasis; Heat-clearing and detoxifying effect; Stopping bleed; The fruit is edible
Rubus corchorifolius L. f.
Stem, Leaf, Fruit
Promoting blood circulations; Stopping bleed; Dispelling wind and cold; Removing dampness; The fruit is edible
Rubus ellipticus Sm.
Young stem, Leaf
Anti-swelling agent; Relieving pain; Antidiarrheic; The fruit is edible
Rubus xanthocarpus Bureau & Franch.
Anti-inflammatory effects; Relieving pain; The fruit is edible
Rubus niveus Thunb.
Dispelling wind and cold; Removing dampness; Clearing away heat and toxic materials; Dysentery; The fruit is edible
Sargentodoxa cuneata (Oliv.) Rehder & E.H. Wilson
Clearing away heat and toxic materials; Promoting blood circulations; Dredging collaterals; Dispelling wind and cold; Convulsive disease
Schisandra propinqua (Wall.) Baillon
Clearing away heat and toxic materials; Anti-swelling agent; Eliminating stasis to stop pain; Stopping bleed
Schisandra propinqua subsp. sinensis (Oliv.) R.M.K. Saunders
Stem, Leaf, Fruit
Dispelling wind and cold; Promoting blood circulations; Clearing away heat and toxic materials; Anti-swelling agent; Relieving pain; Irregular menstruation
Smilax trinervula Miqual
Solanum violaceum Ortega
Anti-inflammatory effects; Clearing away heat and toxic materials; Relieving pain
Symplocos paniculata Miq.
Clearing away heat and toxic materials; Removing blood stasis; Dispelling wind and cold
Taxus wallichiana Zucc. var. chinensis (Pilg.) Florin
Digestion; Ascariasis; Ornamental
Tirpitzia sinensis (Hemsl.) Hallier f.
Anti-swelling agent; Relieving pain; Bone fracture; Ornamental
Toona sinensis (A. Juss.) M. Roem.
Cold; Removing dampness; Stomachache; Dysentery; The leaves is cooked as vegetable
Verbena officinalis L.
Promoting blood circulations; Eliminating stasis to stop pain; Malaria; Clearing away heat and toxic materials; Diuretics; Anti-swelling agent
Wrightia laevis Hook. f.
Traumatic injury; Stopping bleed; Dye plant
Zanthoxylum bungeanum Maxim.
Digestion; Relieving pain; Insecticidal; Anti-itch; Spice
Results and discussion
Diversity of plants used as starters for preparing fermented beverages
Documentation of local wild floristic diversity used by Shui communities as starters for the preparation of fermented beverages is important to support conservation and cultural revitalization efforts in the study site communities. All of the 103 plant species documented as starters are wild harvested and reflect the rich biological diversity in the study area. The greater use of herbaceous plants compared to other types of plants such as woody plant species might be due to their ease of collection, higher abundance, and high effectiveness in comparison to other life-forms. Previous ethnobotanical studies have found that weedy and herbaceous plants are often the majority of plants collected for food, medicine, and other purposes . In addition, various plant parts are use for fermentation with the leaf being the most prevalent plant part used. Previous research in minority socio-linguistic communities in China has shown similar results of leaves being the most prevalent part harvested; this might be due to ease of collection and less threat presented to harvested resources as the practice of harvesting the whole plant or roots presents greater threats to survival [28–30]. In addition, the prevalence of collecting herbaceous plants versus other plant types might also be due to socio-cultural beliefs and practices of the Shui as well as environmental factors. Communities may harvest shrubs, trees, and lianas for their ability to withstand long dry seasons that result in their availability throughout the year as well as increased availability during the harvest period that wild plants are generally collected for starters. Seasons when plants bloomed and fruited, typically during the summer, are also when it is easiest to identify plants for collection. Furthermore, the summer is the best season for microbial fermentation of food and beverages because of the higher temperature promotes fermentation activity. Preparation during the summer also fits within the local Shui calendar as this is after the harvest of rice that rice is used as a raw material input for fermented beverages.
Multiple uses and economic value of the wild plants
Types of multiple uses for wild plants in Sandu County of Guizhou
Informants reported that fermented alcoholic beverages prepared from wild plants had enhanced taste that is smoother compared to beverages prepared without these plants. Most informants reported that using a greater number of plant species as a starter results in improved quality of the final fermented alcoholic beverage. In addition, informants reported that wild species are conveniently located near households in the study area and are available free of cost. Nowadays, informants travel several dozens of kilometers to procure these plants as not all plants used are located near their households and because of habitat destruction near their households. Compared to cultivated plants, wild plants require less management, are usually not directly impacted by agro-chemical pollution, and are a rich source of micronutrients . Interviews and participant observation in local fields [31, 32] found that none of the documented plants were cultivated. In addition, participant observation in local markets  found that none of the documented plants were sold for liquor fermentation.
Traditional harvest and preparation of Jiuqianjiu
Shui families are able to provide the daily quantity and quality of Jiuqianjiu liquor needed from locally available resources that are relatively cheap as well as with the use of simple equipment. Given the accessibility of needed plant material and affordability of equipment in making fermented alcoholic beverages, Shui women can support the social life of their families with this traditional practice. Preparation starts with harvesting wild plants. Harvested plants are usually dried in the sun after collection and are then powdered and stored in a cool dry place for preservation of flavor and health attributes.
The Shui have developed their traditional production sequence of liquor-making according to their farming seasons. Wild plants for starters are harvested between May to September in the study areas. Usually, local households make the beverage starter in summer and brew the liquor during the autumn. The Shui practice of preparing starters in the summer in congruent to previous studies that widely supported that microorganisms bred more quickly in summer and thereby shorten the production cycle and provided greater liquor yield [34, 35]. That is because the temperature in summer is believed to be better due to certain criteria, such as rapid fermentation.
Locals harvest wild plants according to local socially-negotiated protocols that prevent overharvesting of common resources including only harvesting mature individuals when whole plants are needed. In addition, locals prepare enough liquor fermentation starter at one time for three years to ensure plenty of time for plant growth. Local practices of regulating harvesting of common wild plant resources and making enough liquor starter at a time for three years to allow time for plant growth highlights the sustainability ethos of local communities. It is vital to conserve these wild plants while protecting their habitats. They must also be managed in sustainable ways to promote their use in suitable method . It is suggested recruiting ethnobotanists and experts to train local communities on the sustainable utilization of wild plant resources to complement traditional practices [37, 38].
Both non-sticky rice and sticky rice are used by informants as the primary ingredient of Jiuqianjiu liquor with Xiaoqu being the starter agent during the fermentation process. Glutinous rice is the favored rice for liquor brewing by Shui informants because locals perceive it results in a higher quality product that has better taste. There are 15 glutinous rice varieties in Shui communities in Sandu County compared to 7 varieties in other areas of Sandu County. However, the lower yield of glutinous rice results in a relatively higher cost than common rice in the process of liquor making. These glutinous rice varieties are generally reserved for use in festival foods and desserts as well as serving as the main raw material for liquor making in the study area. Locals especially value black glutinous rice. Previous nutrition analysis and phytochemical investigation on black glutinous rice has relatively higher levels of polyphenols and anthocyanin content compared to other varieties of rice; these compounds are known for their medicinal functions in humans including antioxidant activity, reducing cholesterol levels and inhibiting cancer cell proliferation [39–44]. In addition, black glutinous rice has a number of nutritional advantages compared to many other rice varieties including higher content of protein, vitamins and minerals .
Polished rice is used for preparing Jiuqianjiu liquor that is thought to remove substances in rice aside from the starch that are regarded as undesirable substances for liquor brewing. Following polishing, the rice is washed and immerged using cold spring water at room temperature for 24 h. After steeping, excess water is drained off for 4 h before cooking the rice with steam for 1 h in a wood rice steamer. Subsequently, the steamed rice is spread out for cooling in the open air until the temperature falls to nearly 35 °C.
Informants reported a general method for mixing material for starters that includes boiling the plant mixture powder (5 %, w/w) with the spring water (48 %, w/w) for 3 h and then mixing the rice bran and glutinous rice flour (45 %, w/w) with the last year’s starter (2 %, w/w). The grain mix is then pulverized and the plant materials are stirred in. A wild type microbe inoculation method is used to promote the growth of molds, yeasts and other bacteria. The mixed material is typically milled and pressed into a mould of egg-size by hand. Then, it is incubated at 28-30 °C for 7 days in a room with special climatic conditions. After 7 days of incubation the mixture is dried at 45 °C until the humidity is lower than 15 % (w/w). The starter is then stored in a cold and dry place until use for liquor making within the next three years.
The steamed rice is mixed with 1 % (w/w) of the starter mixture that starts the processes of saccharification, acidification, and fermentation of the steamed rice. Then, the mixture is placed in wooden cask that is placed in a pit dug in the ground at 40 °C for 2 days. The saccharified mixture is then mixed with 120 % (w/w) cold spring water to form the thick slurry. Subsequently, the slurry is fermented at room temperature for 15 days in a semi-solid state. This slurry is carefully stirred by local liquor makers to aerate and maintain an optimal level of oxygen and carbon dioxide in the mixture, as well as to maintain an even temperature throughout the fermenting process. After fermentation, the rice wine mash is filtered using a bamboo basket for the purpose of removing the fermented grains. Then, a part of filtered liquid is evaporated to the dense liquid while the other is used for distilling the liquor. The two parts are then mixed at a certain proportion to increase the concentration of alcohol in the mash and halt the fermentation process.
Shui drinking culture and use of Jiuqianjiu
Preparation and consumption of Jiuqianjiu liquor is an important part of traditional social life and interpersonal relations at the study sites. There is a cultural practice to socially consume fermented alcoholic beverages in Shui communities that is known as zhuan zhuan jiu. This practice proceeds by all participants having a pot filled with local liquor on the right hand and feeding this beverage to their neighbor to the right. Informants reported that this practice reflects their respect to guests and is offered as a gesture of equality between the hosts and the guests.
Another use involving Jiuqianjiu liquor is by mothers after giving birth to improve their postpartum recovery. After the third day, mothers can go back to work outdoors if they drank Jiuqianjiu liquor after delivery. In comparison, mothers are commonly not allowed to work until 1 month after birth in China. All the informants indicated that traditional Jiuqianjiu liquor can make women during their puerperium (approximately 6 weeks after birth) and their babies healthy and strong. Informants believe that wild plants play a role in this function. This finding of the role of wild plants after birth is consistent with the findings of Yao people in China, whereas the difference is the mode of administration between Yao people and Shui people. Yao people use medicinal plants for medicine bath whereas the Shui consume these plants via fermented alcoholic beverages [38, 46, 47].
Gender and knowledge transmission
Women are the primary harvesters of plants used as starters for making Jiuqianjiu liquor as well as the makers of these beverages at the study sites. While most of the male Shui informants carry out housework such as cooking, few male informants had knowledge of how to collect plants for starters and prepare fermented alcoholic beverages. Knowledge of plants used as starters is orally transferred from mother to daughter.
Demographic profile of informants
Scale of production
Shui informants prepare fermented beverages for household consumption and commercially in small-scale local distilleries. Small-scale liquor distilleries are distributed widely throughout the study sites and surrounding areas that provide local people with high-quality and relatively affordable local liquor. Wild plants are collected around residences of producers both for when they are preparing beverages for household use as well as for sale in the market. Jiuqianjiu liquor, like most other local alcoholic beverages in minority areas in China, is still produced with traditional equipment that is not backed by scientific knowledge of the process and has little regard for hygiene. Whereas small-scale manufacture has the advantages of short distribution lines, income generation for families, etc., urbanization and increased regional tourism and the resulting growing demand for ready-to-consume high-quality foods requires larger-scale controlled industrial production.
The final products are dependent on local climatic conditions and therefore the sensory characteristics and the quality are variable. The major problem associated with the traditional method of producing Jiuqianjiu liquor is that the product can never be uniform nor predicted because of the diversity of involved microorganisms and difficulties in controlling this population. Upgrading of traditional home-scale processes is needed so that it will lead to a better standardization and safety of a product that is already acceptable to the cultural tastes of local communities. In addition, this standardization process is helpful to continue to maintain and strengthen cultural heritage of Shui communities while providing a product that can compete successfully with industrial versions. However, Jiuqianjiu liquor is a homemade activity and uncontrolled microbial inoculation based on spontaneous fermentation. The ecosystem in Jiuqianjiu liquor represents a source of biodiversity that can be exploited to create such functional starter cultures. The microbiological details of this highly sophisticated fermentation technology has remained undocumented. In fact, Jiuqianjiu liquor has the potential to become an important source of new valuable microbial strains for biotechnology. Future studies are needed to evaluate the phytochemical profiles, bioactivity, stability and safety of fermented wild plants and their synergies.
Wild plants are threatened by various socio-ecological reasons including climate change, land use change, habitat destruction, overharvesting and etc. [48–52]. The construction of highways and other infrastructure as well as deforestation for agricultural purposes is severely threatening wild habitats for plants in Guizhou Province. In addition, unsustainable harvest of plant species with market value also contributes to a decrease of these wild resources. Both ex situ and in situ conservation methods are needed in the natural or farmed environment to preserve the biodiversity of wild plants used for preparing fermented beverages and associated cultural systems. It is necessary that local people support and participate in these conservation initiatives for the most successful results.
This paper provides documentation of the diversity of wild plants used as starters to prepare fermented beverages by Shui communities of Sandu County in Southwest China. Findings highlight the rich biodiversity and habitats that local communities draw upon from their surroundings as part of their cultural life to support interpersonal communication and celebrate key occasions. Women’s role as the primary producers involved in making fermented beverages reflects gendered knowledge that is related to societal life and relations to kin. While knowledge of plants used for liquor making has traditionally been orally transferred from mother to daughter, this knowledge is threatened as the younger generations move away from rural areas in search of jobs and a different lifestyle, a pattern witnessed in rural communities worldwide. Efforts are needed to enhance the transmission of ethnobotancial knowledge in Shui communities towards conservation of biodiversity and associated preservation of cultural systems. Increased interest in natural products and artisanal beverages as well as increased regional tourism is attracting new interest in wild plants used in the processing of fermented foods and beverages. If developed with local community interests and conservation in mind, these commercialization and tourism efforts have the potential of helping preserve traditional ethnobotanical knowledge as well as associated biodiversity. Future studies are needed to evaluate the phytochemical profiles, bioactivity, stability and safety of fermented wild plants and their potential for other fermented foods and beverages as well as medicinal purposes. In addition, it is necessary to develop standards for large-scale production and commercialization of these non-timber forest products. These future studies would help provide guidelines for community-based production and ultimately preservation of biological and cultural diversity.
Permissions were provided by all participants in this study, including the Shui people. Consent was obtained from the participants prior to this study being carried out. The authors have all copyrights.
We are grateful to Shui people in Sandu County of Guizhou Province for their assistance in the field investigations and for sharing their valuable knowledge. Our research team is also grateful for the numerous discussions and field trips with members of the Ethnobotanical Laboratory at Minzu University of China and the Research Group of Ethnobotany at Kunming Institute of Botany, Chinese Academy of Sciences, and Kaili University, and Guizhou Normal College. We would like to thank Ling Wang from the Department of Minority Languages and Literatures at Minzu University of China for correcting and typing the Shui names of wild plants used for starting fermented beverages. This study was funded by the Ministry of Science & Technology of China (2012FY110300), the National Natural Science Foundation of China (31161140345 & 31360070), the Ministry of Education of China and the State Administration of Foreign Experts Affairs (B08044), and Minzu University of China (MUC 10301-01404031-84 & YLDX10103).
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