Integrating ethnobiological knowledge into biodiversity conservation in the Eastern Himalayas
© The Author(s). 2017
Received: 30 December 2016
Accepted: 21 February 2017
Published: 29 March 2017
Biocultural knowledge provides valuable insight into ecological processes, and can guide conservation practitioners in local contexts. In many regions, however, such knowledge is underutilized due to its often-fragmented record in disparate sources. In this article, we review and apply ethnobiological knowledge to biodiversity conservation in the Eastern Himalayas. Using Sikkim, India as a case study, we: (i) traced the history and trends of ethnobiological documentation; (ii) identified priority species and habitat types; and, (iii) analyzed within and among community differences pertaining to species use and management. Our results revealed that Sikkim is a biocultural hotspot, where six ethnic communities and 1128 species engage in biocultural relationships. Since the mid-1800s, the number of ethnobiological publications from Sikkim has exponentially increased; however, our results also indicate that much of this knowledge is both unwritten and partitioned within an aging, gendered, and caste or ethnic group-specific stratum of society. Reviewed species were primarily wild or wild cultivated, native to subtropical and temperate forests, and pend IUCN Red List of Threatened Species assessment. Our results demonstrate the value of engaging local knowledge holders as active participants in conservation, and suggest the need for further ethnobiological research in the Eastern Himalayas. Our interdisciplinary approach, which included rank indices and geospatial modelling, can help integrate diverse datasets into evidence-based policy.
Conservation practitioners have historically considered the role of human communities only or primarily in terms of the threats that extractive and transformative activities pose on the environment [1–3]. As a theoretical consequence, people-free or ‘fortress conservation’ strategies have become the dominant means of protecting ‘natural’ systems from anthropogenic influence [4, 5]. However, over the past two decades, a paradigm shift among conservationists has challenged this convention [6–8]. Termed biocultural approaches to conservation , recent programs have integrated the innovations, practices, and worldviews of Indigenous and local communities into policies addressing the rapid attrition of Earth’s biological and cultural diversity, hereafter termed biocultural diversity [9–12].
Thematically, biocultural approaches to conservation emphasize the dynamic, multi-scalar feedback loops that link social and ecological processes . They synthesize biodiversity science and ethnographic fieldwork to discern processes that shape extant Earth systems . In doing so, they help deconstruct the dualism separating ‘nature’ from society, and place local people back in parks as conservation agents [14–16]. Worldwide, such projects have had a variety of reported successes, including heightened spiritual connection and increased environmental literacy [16, 17]. However, debates continue as to the verity of reported claims and the extent to which conservation programs should serve human welfare .
‘Landscape’ initiatives in the Eastern Himalayas evidence the successful utilization of biocultural principles for conservation purposes . In 1997, an International Centre for Integrated Mountain Development (ICIMOD)-led collective petitioned for the designation of Mt. Khangchendzonga as a dynamic complex of socio-ecological interaction [19, 20]—a biocultural hotspot. The transboundary Khangchendzonga Landscape (KL) is situated within the Himalayan Biodiversity Hotspot [21, 22], and includes Bhutan, India, and Nepal. It incorporates 7.2-million people belonging to diverse ethnic communities, including Indigenous groups like the Lepchas of Sikkim and Darjeeling, the Lhop (Doya) of Amu Mo Chhu Valley, and Walungpas of Walangchung Gola of Taplejung . Because of this complexity, biocultural approaches to conservation facilitated environmental management in the KL. Co-management, community-based conservation, and integrated conservation and development, for example, have empowered Indigenous and local peoples through non-government organizations (NGOs), and promoted international cooperation along sensitive geopolitical boundaries [21–24].
Protected areas (PAs) in the Sikkim Eastern Himalayas, and the potential number of species with ethnobiological records found in each based on reviewed altitudinal range data
Altitudinal Range (m)
Estimated Species with Ethnobiological Records
Khangchendzonga National Park
Shingba Rhododendron Sanctuary
Maenam Wildlife Sanctuary
Fambonglho Wildlife Sanctuary
Kyongnosla Alpine Sanctuary
Barsey Rhododendron Sanctuary
Kitam Bird Sanctuary
Pangolakha Wildlife Sanctuary
Traditional and community knowledge buttresses conservation policies in Sikkim and is heralded for its adaptive capacity. However, at the same time, Sikkim’s biocultural heritage is threatened by ‘modernizing’ forces associated with globalization and rapid climatic change. As noted in the Sikkim Biodiversity Action Plan, the state lacks formalized and collated records of its biodiversity, which extends into ethnobiological documentation . Even among existing studies, including ethnobiological datasets, records are strictly qualitative, and exist as repetitive, fragmentary notes that lack a consolidated attempt for strengthening policy [28, 29]. Mobilizing this knowledge and associated datasets into environmental management programs remains a challenge.
In this review, we explore the application of ethnobiological knowledge for biodiversity conservation in Sikkim. Specifically, we ask: (i) What is the spatio-temporal pattern of ethnobiological knowledge documentation?; (ii) How is ethnobiological knowledge partitioned within and among ethnic communities?; (iii) What species are priority targets for conservation, and are these species found within protected areas? In asking these questions, we hope to reframe discourses that focus on the Eastern Himalayas as only or primarily a reservoir of biological and genetic diversity. To our knowledge, our manuscript serves as the first ethnobiological review of the Sikkim Eastern Himalayas.
The Sikkim Eastern Himalayas
Sikkim is divided into four districts, and situated between Bhutan, Nepal, the Tibetan Autonomous Region of China (TARC), and the Indian State of West Bengal. In the 17th Century, Lepcha and Bhutia communities established Sikkim as a Buddhist monarchy under kings termed Chogyals. Chogyals ruled for approximately 350 years until multi-directional process of change resulted in Sikkim’s protectorate status and eventual integration into India as its 22nd state in 1975 . Prior to integration, major socio-ecological changes followed contact with the British East India Company in the mid-1880s. During this period, Nepali migration, here a generic term that includes many castes and ethnicities, was incentivized to promote colonial agricultural development in the Eastern Himalayas . These progressive changes resulted in a rich admixture of ethnobiological traditions from the Greater Himalayas.
Demographic records from Sikkim have varied in quality since the first census in 1891; notwithstanding, Sikkim’s population appears to have increased from 30,458 to 607,688 people between 1891 and 2011 [32, 33]. The Anthropological Survey of India identified 25 ethnic communities in the state during its first ethnographic survey between 1988 and 1990 . These communities are generally grouped as: (i) Bhutias (Lhopos, including Denjongpas, Lachenpas, and Lachungpas) and Lepchas, the autochthons of Sikkim who represent less than 20% of the total population; (ii) People of Nepalese origin, mainly Limbus and Rais, who began migrating to Sikkim from the 1870s and represent more than 75% of the population; and, (iii) People from the plains of India, including Bengalis, Biharis, and Marwaris . In June 1978, Lepcha, Bhutia, Chumbipa, Dopthapa, Drokpa, Kagate, Sherpa, Tibetan, Tromopa, and Yolmo communities were recognized as Scheduled Tribes in Sikkim; the Kami, Damai, Lobar, Majhi and Sarki were classified as Scheduled Castes. The Government of India considers some ‘Nepali’ identifying or identified groups in Sikkim as ‘backward castes’: Gurung, Magar, Newars, Limbu/Subba, Rai, Sunwar, and Tamang. Bengali, Bihari, Deswali, Marwari, and Punjabi -identifying communities, all recent migrants who are diverse both within and among respective communities, are well-established in modern Sikkim . In total, our study recognizes 32 ethnic communities, 17 languages, and 9 religions in Sikkim [34, 36–38].
Some characteristic taxa with ethnobotanical records
Tropical (<1000 m)
Tropical riverine evergreen/deciduous Forest
Bombax ceiba, Cycas pectinata, Dalbergia sissoo,
Tropical Moist Evergreen/Deciduous Forest
Dillenia indica, Duabanga grandiflora, Garuga pinnata,
Tropical Moist Mixed Forest
Lagerstromia speciosa, Mimosa pudica, Shorea robusta
Tropical Dry Evergreen/Deciduous Forest
Subtropical (1000–2000 m)
Subtropical Riverine evergreen/Deciduous Forest
Callicarpa arborea, Castanopsis tribuloides, Fraxinus floribunda,
Subtropical Moist Evergreen/Deciduous Forest
Macaranga pustulata, Mangifera sylvatica, Pandanus furcatus,
Subtropical Moist Mixed Forest
Saurauia nepaulensis, Schima wallichi
Subtropical Dry Evergreen/Deciduous Forest
Warm Temperate (2000–2500 m)
Warm Temperate Riverine Evergreen/Deciduous Forest
Alnus nepalensis, Castanopsis tribuloides, Engelhardia spicata,
Warm Temperate Moist Evergreen/Deciduous Forest
Evodia fraxinifolia, Ilex dipyrena, Juglans regia,
Warm Temperate Moist Mixed Forest
Lithocarpus pachyphyllus, Quercus lamellosa, Zanthoxylum acanthopodium
Warm Temperate Dry Evergreen/Deciduous Forest
Cool Temperate (2500–3000 m)
Cool Temperate Riverine Deciduous Forest
Acer caudatum, Betula utilis, Cinnamomum impressinervium
Cool Temperate Moist Evergreen Forest
Cryptomeria japonica, Magnolia lanuginosa, Mahonia sikkimensis,
Cool Temperate Moist Mixed Forest
Rhododenron arboreum, Quercus lineata
Cool Temperate Dry Evergreen Forest
Subalpine (3000–4000 m)
Subalpine Riverine Evergreen Forest
Abies densa, Abies spectabilis, Berberis insignis, Juniperus recurva,
Subalpine Moist Evergreen Forest
Larix griffithiana, Rhododenron barbatum, Rhododendron campanulatum, Taxus wallichiana
Subalpine Moist Deciduous Forest
Subalpine Dry Evergreen Forest
Alpine (>4000 m)
Juniperus indica, Rhododendron fulgens, Rhododendron nivium
Data collection and standardization
From October 2015 through February 2016, we conducted a systematic review of publically available and accessible literature pertaining to ethnobiological knowledge in the Sikkim Eastern Himalayas. For this study, we defined ethnobiological knowledge as traditional and community knowledge—Indigenous and non-Indigenous—related to socio-ecological interactions between identified or identifiable taxa and the people of Sikkim. Using search terms Darjeeling/Kalimpong/Sikkim/Eastern Himalaya AND Ethno/Indigenous/Traditional, we searched four digital databases: (i) ENVIS ; (ii) Google Scholar; (iii) NELUMBO ; and, (iv) Project Muse . We included Darjeeling and Kalimpong (West Bengal, India) as place-based keywords due to their historical association with the Kingdom of Sikkim. After analysis, we omitted data published in the ENVIS Medicinal Plants of Sikkim database due to its primary reference of non-Sikkimese user groups and medical traditions. We then conducted archival research at six institutions in Gangtok, Sikkim using the same criteria: (i) The Botanical Survey of India; (ii) The G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Sikkim Unit; (iii) The Namgyal Institute of Tibetology; (iv) Home Department, Government of Sikkim Central Library; (v) Sikkim University Central Library; and, (vii) Sikkim State Bioinformatics Institute. Once collected, each source was reviewed for the following subsets of data: study site name, including the names of sacred landscapes, cities, villages, panchyats, samitis, blocks, districts, and subdivisions; bio-physical characteristics of site-specific studies; publication date; Indigenous and local castes, clans, and groups surveyed; and, species diversity. These sources are provided as an additional file [see Additional file 1].
We transcribed species data from each reviewed record into a working database [see Additional file 2]. After all sources were reviewed, we then standardized species to current taxonomic designations using international databases and field guides [43–48]. Concurrently, we tabulated the relative citation frequency for each species, and partitioned uses into one of 19 accepted categories (Table 2) [49, 50]. Relative citation frequency was calculated by dividing each citation value by the value of the most frequently cited species [see Additional file 2]. Regarding ecological data, we detailed Sikkim-specific altitudinal range data when possible [51–58]; data from the region were used as a proxy in the absence of Sikkim-specific records [45–47, 59–61]. Finally, we collected the following data: the conservation status of species from the IUCN Red List of Threatened Species  and Government of Sikkim ; and, naturalization, cultivation, or domestication status [47, 64]. Our study assumes that the number of use categories reported for a given species corresponds with the amount of attention it receives from communities in Sikkim. It is important to note that the number of uses might not correspond to current and active applications of those uses.
We geo-referenced reported study sites and conducted spatio-temporal analyses of reviewed data in ArcGIS [11, 65, 66]. Specifically, we performed the following assessments: (i) identification of administrative districts with the highest representation of ethnobiological records; (ii) temporal analysis of ethnobiological knowledge documentation; and, (iii) identification of surveyed communities and their knowledge documentation through time.
Criteria for ranking species for Sensitivity Rank (SR) of reviewed species
High Relative Intensity (D)
Harvesting/utilizing either (i) whole animal or animal part in a manner that reduces animal’s lifespan (i.e. bones, ivory, meat, etc.); or (ii) whole plant, rootstock, rhizome, fungal body, etc.
Low Relative Intensity (N)
Species engagement excluding the above
Range limited to one habitat zone
Range extending to two or more habitat zones (Refer to Table 2 for habitat zones)
IUCN or Government of Sikkim-recommended Critically Endangered (CR), Endangered (EN), or Vulnerable (VU)
Not Threatened (U)
IUCN or Government of Sikkim-recommended Near Threatened (NT), Least Concern (LC), or Not Assessed (NA)
Using the altitudinal range of each reviewed species, we modeled biocultural hotspots in Sikkim using a standard methodology at 100 m altitudinal resolution (Fig. 2) (see ). Here, we aimed to project a qualitative map that identified priority regions for biodiversity conservation based on the altitudinal range of reviewed species. We acknowledge that modeling procedures often account for GIS-based, site-specific occurrences and bioclimatic variables associated with specific species [70, 71]. However, such data from Sikkim is only available in heterogeneous, fragmented forms that are geographically biased or incorrect. Moreover, Sikkim’s topography, which averages 40° slope, and altitudinal variation, ranging from 284 m to 8586 m, generate a plethora of unpredictable microhabitat and microclimatic conditions that: influence species distributions; limit the practicality of field surveys; and, bias conventional modeling procedures . Therefore, in the absence of data, our model engages altitudinal distribution data as the sole proxy for various methodologies .
Surveyed ethnic communities
Six of 32 identified ethnic communities in Sikkim have written ethnobiological records: Bhutia, Lepcha, Limbu, Nepali, Sherpa, and Tibetan communities. However, most reviewed records were not ethnic-group specific and reported data and/or knowledge in Sikkim-vernacular Nepali language. Nepali-identified or identifying groups, including non-specific reports detailed in Nepali language, had the greatest number of identified species (732), followed by Lepchas (377), Limbus (298), Tibetans (120), Bhutias (74), and Sherpas (35). Four categories of male specialized users and spiritual healers were reported from four ethnic communities: Bhutia: Lama; Lepcha: Bomthing, Mon-bomthing; Nepali: Bijuwa, Fedangwa, Jhakri; and, Tibetan: Amchis.
A total of 1128 species distributed across three kingdoms, 213 families, and 712 genera have ethnobiological records in the Sikkim Eastern Himalaya [see Additional file 2]. Plantae was the most surveyed kingdom (995 species; 625 genera; 160 families), housing 88% of all reviewed species. Animalia (species: 76; genera: 50; families: 28; 7% of reviewed species) and Fungae (species: 57; genera: 37; families: 25; 5% of reviewed species) exhibited a comparative dearth of records [see Additional file 2]. Across kingdoms, 105 species were considered Himalayan or Eastern Himalayan endemic [45, 79]. The average range amplitude of species in Animalia was 1098 m (+/- 1009 m SD), in Fungae 1683 m (+/- 884 m SD), and in Plantae 1329 m (+/- 584 m SD).
In Plantae, angiosperms were the most explored discipline, accounting for 96% of all documented plant species (957). Pteridophytes (23), Gymnosperms (10), and Bryophytes (5) received notably less attention in comparison . Five plants were reported as endemic to the Sikkim Eastern Himalayas, namely: Aconitum ferox var. naviculare, Allium sikkimense, Mahonia sikkimensis, Rhododendron sikkimensis, and Swertia pedicellata. The taxonomic designations for these and many of our reported species, however, are pending official revision. In Animalia, fish were the most represented Chordates (37), followed by Mammals (22), Birds (12) and Amphibians (3) [see Additional file 2]. Three invertebrates were cited, two of which were in the Apidae. In Fungae, Basidiomycetes accounted for 77% of reviewed species (44), with approximately 85% of the remaining 13 Ascomycetes being Lichens.
The distribution of reviewed species across 19 ethnobiological categories
Treatment for Animal Venom, Fish Stupefying
Behavioral and Mental Health
Hysteria, Mental and Nervous Disorders
Bleeding, Blood Health, Hemorrhage, Lymphatic System
Antiseptic, Dermatological Health
Abrasions, Burns, Boils, Skin Diseases and Parasites, Hair Problems
Dental and Oral Health
Throat Infection, Toothache, Oral Infection
Ear and Mastoid Health
Earache, Hearing Deficiency, Vertigo
Endocrine and Metabolic Health
Diabetes, Hormonal Disorder
Diarrhea/Dysentery, Indigestion, Laxative, Nausea, Parasites, Vomiting
Bladder and Renal Infections, Sexually Transmitted Infections, Urinary-tract Infections
Jaundice, Liver Disorders
Antispasmodic, Body Aches, Sprain, Fracture, Rheumatism/Arthritis
Cancer, Growths, Moles, Tumors
Nervous System Health
Epilepsy, Memory, Migraine, Stimulant
Adnexa, Eye Disease
Pregnancy, Childbirth, Perinatal Care
Delivery, Lactation, Menstruation, Pregnancy
Allergy, Asthma, Bronchitis, Pneumonia,
Edible, Food, Butter, Oil, Flour, Pickle, Dry Fruit, Candies
Cultural or Spiritual Use
Culture-specific Disease, Folktales, Legends, Ritual Ingredients, Spirituality
Art, Construction, Dye, Fodder, Handicrafts, Instrument, Utensils
Wild (CR 5 and 3; 922 species; 82% total) and native taxa (CR 5; 817; 72%) were cited more frequently than wild-cultivated (CR 4 and 2; 107; 10%) and cultivated species (CR 1; 99; 8%). Most species were harvested, cultivated, or used in a sustainable manner (SR 1-4; 642; 57%) and exhibited altitudinal distributions that crossed multiple habitat types (SR 6-5, 21; 962; 85%). Of 1128 species, approximately 80 species were both destructively harvested and had restricted ranges. Four of these species also had a threatened status in Sikkim (SR 8), including Cymbidium grandiflorum (Orchidaceae), Flickingeria fimbriata (Orchidaceae), Ophiocordyceps sinensis (Ophiocordycipitaceae), and Tor putitora (Cyprinidae). Based on IUCN Red List of Threatened Species assessments , three of all species were Critically Endangered (CR), four are Endangered (EN), seven are Vulnerable (0.64; VU), 11 are Near Threatened (1.00% NT), 99 are of Least Concern (9.03% LC), and 972 species have not been assessed (88.69% NA) [see Additional file 2]. An additional 25 species have recommended conservation statuses by the Government of Sikkim based on IUCN-CAMP criteria (; see Additional file 3). These species include exploited medicinal plants such as Swertia chirayita, Nardostachys jatamansi, Picrorhiza kurroa (Plantaginaceae), Sinopodophyllum hexandrum (Berberidaceae), and Valeriana jatamansi (Caprifoliaceae).
Our review indicates that Sikkim has a rich biocultural heritage that includes knowledge pertaining to over 1100 species of animals, fungi, and plants. Local people not only know about the useful properties of these species, but also the community ecology and life histories of diverse organisms . These aspects of ethnobiological knowledge, which encompass abundance, distribution, and phenology, significantly influence community management practices and can therefore benefit conservation planning in Sikkim [29, 79]. For instance, in previous studies, Lepcha communities were engaged by government researchers to understand the population status of under-surveyed bird species; local communities were found to provide “data” at the accuracy needed to make management decisions [81, 82]. Our reviewed records also implied that faith traditions and community taboos sustain many ethnobiological relationships in Sikkim, and cultivate a sense of stewardship toward critical habitat [35, 83–86]. We conclude that targeting biocultural knowledge systems, including gaps in ethnobiological research, is a practical way to incorporate local peoples—their knowledge, land, and participation—into multi-scalar conservation directives in the Eastern Himalayas.
Our analyses illuminated the dynamic nature of ethnobiological knowledge, and evidenced its ongoing construction amidst changing socio-ecological conditions. We traced a dramatic increase in ethnobiological records published since the 1950s, with a significant spike in the mid-1990s. This trend appears correlated with both the relaxation of permit restrictions into Sikkim, and the 1993 initiation of the Convention on Biological Diversity (CBD) following the Earth Summit in Rio de Janeiro in 1992. The CBD obliged signatory polities, including India, to acknowledge and preserve biocultural knowledge as an adaptive resource for conservation initiatives, record and disseminate biocultural knowledge for practical applications, and ensure equitable benefits arising from biocultural knowledge (reviewed by ). Our assessment highlighted, however, a stark difference in ethnobiological knowledge within and among ethnic communities. Intra-cultural differences compounded overall knowledge diversity based on age, gender, occupation, and individual strategies and interests . These factors were not quantitatively justified in any of our reviewed studies. The paucity of records from 26 identified ethnic communities further indicates that much of Sikkim’s biocultural heritage remains as unwritten, oral traditions situated within a gendered, caste-specific, and aging stratum of society. In the future, we suggest that researchers begin addressing these gaps through collaborations with nomadic or semi-nomadic peoples at high altitudes, including Bhutia, Chumbipa, Dopthapa, Drokpa, Kagate, Sherpa, Tromopa and Yolmo communities. High-altitude zones are particularly sensitive to climatic changes that may alter community assemblages, ecological processes, and, as an extension, historical ways of relating to the environment [85, 87, 88]. With the participation of these groups, conservationists can craft more holistic and culturally appropriate strategies for both restoration and conservation in the Eastern Himalayas.
As previously alluded to, older members of rural communities were the primary user group of reviewed species, namely for medicine. Knowledge of species use, practice, and folklore was reported to decrease in recent generations, as much of the knowledge was documented from collaborators between 50 and 70 years of age [26, 29]. Today, younger generations migrate to urban centers where they are neither exposed to local species nor the traditions that surround them. Both imposed legal structures in the early 21st Century and market liberalization in the mid-1990s have further complicated knowledge transmission and species use, resulting in the attrition of ethnobiological knowledge in Sikkim. For instance, between the 1970s and 1990s, the Sikkim Forest, Environment and Wildlife Management Department permitted commercial exploitation of medicinal plants, including from PAs. However, as of 2001, the government implemented a 5-year ban on medicinal plant collection via Order No. 13/F/Env&W. This order received a 5-year extension in 2006, and will likely be ratified again in the future. Moreover, local access to state healthcare providers and allopathic medicine has reduced local dependency on wild animals, fungi, and plants as medicine.
Despite the decreased reported use of medicinal plants, many wild species remain a vital part of Sikkimese cuisine [89–94]. Local communities have regular access to wild edibles at markets in the cities of Gangtok (East District), Geyzing (West District), Namchi (South District), and Singtam [91, 93, 95]. Various ferns (Diplazium spp.; Athyriaceae), the Sikkim Cobra Lily (Arisaema utile; Araceae), and Stinging Nettles (Uritica dioica; Urticaceae) were some frequently cited edibles, and were often prepared alongside pickled vegetables, like Nodding Tupistra (Tupistra nutans; Asparagaceae), in traditional Sikkimese cuisine [96–99]. Fruits from Bastard Oleaster (Elaeagnus latifolia; Elaeagnaceae), Burmese Grape (Baccaurea ramiflora; Phyllanthaceae), and Machilus edulis (Lauraceae) were also seasonal favorites with high reported consumption [100–102]. Regarding Fungae, commercial cultivation has increased in lower-altitude zones, particularly of species in the genera Agaricus and Pleurotus [51, 52, 103]. Wild animals were also consumed for medicinal purposes, albeit at low reported frequency and only in rural contexts. The meat of Asian Black Bear (Ursus thibetanus; Ursidae), Bengal Fox (Vulpes bengalensis; Canidae), Central Himalayan Langur (Semnopithecus schistaceus; Cercopithecidae), Himalayan Crestless Porcupine (Hystrix brachyura; Hystricidae), and Hodgson’s Giant Flying Squirrel (Petaurista magnificus; Sciuridae) were reported to treat respiratory diseases, namely Tuberculosis, which are common in Sikkim [87–104]. Fish are available in most market places, and have high reported consumption; however, their populations likely face threats from hydroelectric dam development in Sikkim [105, 106]. Future studies should quantify the value and quantity of wild edibles sold in marketplaces to better understand anthropogenic pressures on wild populations and expand wild-cultivation practices when possible.
Incentivizing and/or commercializing the cultivation of edible and medicinal plants and fungi within agroforestry systems may reduce pressure on wild populations and create habitat corridors for threatened species [29, 107]. To date, most conservation efforts in Sikkim have been directed toward the nominal designation of PAs at mid and high altitudes. Military encampments and agricultural landscapes surround these PAs. Moreover, traditional doctors, spiritual healers, and rural villagers still harvest and collect many reviewed species from these areas despite legal restrictions [104, 108–112]. Conservationists can begin addressing these pressures, without marginalizing local communities, by promoting cooperative agroforestry programs along PA borders. Recent reviews, for example, have suggested integrating edible and medicinal plant cultivation into existing Nepalese Paperbush (Edgeworthia gardneri; Thymelaeaceae) or Black Cardamom (Amomum subulatum; Zingiberaceae) agroforestry systems which already yield high profits [29, 113, 114]. As noted by Charnley et al. , such programs must do more than identify the “right” or best model for knowledge application and sharing, and must address existing societal factors that may hinder program implementation or undermine community structures. Organizational frameworks could, therefore, draw upon pre-existing dzumsa and dwichi committee structures in Sikkim, which have legacies of conservation impact [116, 117]. Any cooperative, however, must ensure that agroforestry systems produce marketable amounts of edibles that can either be preserved or transported to market before spoilage (as reviewed by [29, 116]). Our conservation rank system and additional files is useful for identifying target species based on criteria of interest, including medicinal use, altitudinal range, and population status [see Additional files 1, 2 and 3].
Incorporating ethnobiological knowledge into biodiversity conservation is a meaningful way to empower local communities to both monitor and preserve species of biocultural importance [9, 11, 116]. Based on our review, communities have obvious incentive to conserve biodiversity for cultural purposes and practical use. However, our results suggest a literature bias toward medicinal plants, and a paucity of records from the kingdoms Animalia and Fungae. To hone the applicability of our biocultural hotspot concept, we recommend that researchers incorporate new criteria, including species-specific ranges and habitat information, into our model structure. Moreover, we suggest that researchers document ethnobiological relationships that extend beyond medicinal uses of species to include living oral traditions, folklore, art, etc. By combining ethnobiological surveys with biodiversity science, particularly the gaps noted in recent reviews [21, 29], conservationists can better understand the socio-ecological dynamics shaping modern Sikkim.
We collated and applied ethnobiological knowledge to promote biodiversity conservation in the Eastern Himalayas. We began with a spatio-temporal review of biocultural records from Sikkim, India to understand: (i) patterns in biocultural knowledge documentation; (ii) the diversity of species with biocultural records; and (iii) the partitioning of biocultural knowledge within and among ethnic communities. We then galvanized these records into two conservation indices and a biocultural hotspot model that indicate conservation priorities in Sikkim.
We would like to thank our collaborators at the United States India Education Foundation (USIEF) and the United States William J. Fulbright Commission for their support and inspiration under a Fulbright-Nehru Research Fellowship awarded to ARO at the G. B. Pant National Institute for Himalayan Environment and Sustainable Development (GBPNIHESD). ARO and HKB are highly grateful to the Director of the Institute for providing necessary facilities and support at the Sikkim Unit. We would also like to extend our gratitude to Jhony Lepcha, a Researcher at the Sikkim Unit (GBPNIHESD), for his help generating our manuscript figures. ARO would like to extend gratitude to L. Lepcha, Sikkim State Council of Science & Technology, and T. Cho Cho and P. Gurung, Sikkim State Home Department, for their assistance during our research period.
This research was funded by a Fulbright-Nehru Research Scholarship granted to ARO by the United States-India Education Foundation (USIEF) and the Institute for International Education (IIE). Funding agencies did not participate in any aspect of project design, study implementation, data interpretation, or manuscript preparation.
Availability of data and materials
ARO, HKB, PPD, and SKR participated in study design and implementation. ARO and HKB conceived the study, and ARO prepared the manuscript. ARO, HKB, PPD, SKR collected and standardized data for analysis. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
The authors of this manuscript consent for publication.
Ethics approval and consent to participate
Our study is exempt from IRB approval because it is a review of literature. As such, there was no need to request consent for participation in our study.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Maffi L. On biocultural diversity: linking language, knowledge, and the environment. Washington: Smithsonian Institution Press; 2001.Google Scholar
- McShane TO, Hirsch PD, Trung TC, Songorwa AN, Kinzing A, Monteferri B, et al. Hard choices: making trade-offs between biodiversity conservation and human well-being. Biol Conserv. 2011; doi:10.1016/j.biocon.2010.04.038.
- Sarkar S, Montoya M. Beyond park and reserves: the ethics and politics of conservation with a case study from Peru. Biol Conserv. 2011; doi:10.1016/j.biocon.2010.03.008.
- Wilshusen PR, Brechin SR, Fortwangler CL, West PC. Reinventing a square wheel: critique of a resurgent “protection paradigm” in international biodiversity conservation. Soc Nat Resour. 2002; doi:10.1080/089419202317174002.
- Adams WM, Hutton K. 2007 People, parks and poverty: political ecology and biodiversity conservation. Conserv Soc. 2007; URL: http://www.conservationandsociety.org/text.asp?2007/5/2/147/49228.
- Ellis EC, Ramankutty N. Putting people on the map: anthropogenic biomes of the world. Fron Ecol Environ. 2008; doi:10.1890/070062.
- Doak DF, Bakker VJ, Goldstein BE, Hale B. What is the future of conservation? Trends Ecol Evolut. 2014; doi:10.1016/j.tree.2013.10.013.
- Rotherman ID. Bio-cultural heritage and biodiversity: emerging paradigms in conservation and planning. Biodivers Conserv. 2015; doi:10.1007/s10531-015-10065.
- Gavin MC, McCarter J, Mead A, Berkes F, Stepp JR, Peterson D, et al. Defining biocultural approaches to conservation. Trends Ecol Evolut. 2015; doi:10.1016/j.tree.2014.12.005.
- Maffi L. Biocultural diversity and sustainability. In: Pretty J, Ball AS, Benton T, Guivant J, Lee DR, Orr D, et al., editors. The Sage handbook of environment and society. London: SLE Pound; 2007. p. 267–77.View ArticleGoogle Scholar
- Ens EJ, Pert P, Clarke PA, Budden M, Clubb L, Doran B, et al. Indigenous biocultural knowledge in ecosystem science and management: review and insight from Australia. Biol Conserv. 2015; doi:10.1016/j.biocon.2014.11.008.
- Blanco J, Carriere SM. Sharing local ecological knowledge as a human adaptation strategy to arid environments: evidence from an ethnobotany survey in Morocco. J Arid Environ. 2016; doi:10.1016/j.jaridenv.2015.10.021.
- Loh J, Harmon D. A global index of biocultural diversity. Ecol Indic. 2005; doi:10.1016/j.ecolind.2005.02.005.
- Reed MS. 2008. Stakeholder participation for environmental management: a literature review. Biol Conserv. 2008; doi:10.1016/j.biocon.2008.07.014.
- Berkes F. 2004. Rethinking community-based conservation. Conserv Biol. 2004; doi:10.1111/j.1523-1739.2004.00077.x.
- Berkes F, Colding J, Folke C. Rediscovery of traditional ecological knowledge as adaptive management. Ecol Appl. 2000; http://www.jstor.org/stable/2641280.
- Rutte C. The sacred commons: conflicts and solutions of resource management in sacred natural sites. Biol Conserv. 2011; doi:10.1016/j.biocon.2011.06.017.
- Phuntsho K, Chettri N, Oli K. Mainstreaming community-based conservation in a transboundary mountain landscape: lessons from Khangchendzonga. Kathmandu: International Centre for Integrated Mountain Development; 2012.Google Scholar
- Chettri N, Sharma E. Prospective for developing a transboundary conservation landscape in the Eastern Himalayas. In: McNeely JA, McCarthy TM, Smith A, Olsvig-Whittaker L, Wikramanayake ED, editors. Conservation biology in Asia. Kathmandu: Society for Conservation Biology Asia Section and Resources Himalaya; 2006. p. 2006.Google Scholar
- Chettri N, Shakya B, Sharma E. Biodiversity conservation in the Khangchenjunga Landscape. Kathmandu: International Centre for Integrated Mountain Development; 2008.Google Scholar
- Kandel P, Gurung J, Chettri N, Ning W, Sharma E. Biodiversity research trends and gap analysis from a transboundary landscape, Eastern Himalayas. J Asia Pac Biodivers. 2016; doi:10.1016/j.japb.2015.11.002.
- ICIMOD, WCD, GBPIHED, RECAST, MoFSC. Kangchenjunga landscape conservation and development initiative, feasibility assessment report regional synthesis. 2015. Draft Report.Google Scholar
- Carlsson L, Berkes F. Co-management: concepts and methodological implications. J Environ Manage. 2005; doi:10.1016/j.jenvman.2004.11.008.
- Salafsky N, Wollenberg E. Linking livelihoods and conservation: a conceptual framework and scale for assessing the integration of human needs and biodiversity. World Dev. 2000; doi:10.1016/S0305-750X(00)00031-0.
- Badola HK, Lepcha J, Gaira KS, Sinha S, Dhyani PP. Socio-economic and bio-resource assessment: participatory and household survey methods, tools and techniques. New Delhi: Highlanders Communications Private; 2016.Google Scholar
- Pradhan BK, Badola HK. Ethnomedicinal plant use by Lepcha tribe of Dzongu Valley, bordering Khangchendzonga Biosphere Reserve, in North Sikkim, India. J Ethnobiol Ethnomed. 2008; doi:10.1186/1746-4269-4-22.
- Government of Sikkim. Sikkim biodiversity action plan. Gangtok: Forest, Environment and Wildlife Management Department; 2012.Google Scholar
- Brook RK, McLachlan SM. Trends and prospects for local knowledge in ecological and conservation research and monitoring. Biodivers Conserv. 2008; doi:10.1007/s10531-008-9445-x.
- Uprety Y, Poudel RC, Gurung J, Chettri N, Chaudhary RP. Traditional use and management of NTFPs in Kangchenjunga Landscape: implications for conservation and livelihoods. J Ethnobiol Ethnomed. 2016; doi:10.1186/s13002-016-0089-8.
- Duff A. Sikkim: requiem for a Himalayan kingdom. London: Random House; 2015.Google Scholar
- Sivaramakrishnan K. Modern forests: statemaking and environmental change in colonial Eastern India. Stanford: Stanford University Press; 1999.Google Scholar
- Turin M. Results from the linguistic survey of Sikkim: mother tongues in education. In: Balikci-Denjongpa A, McKay A, editors. Buddhist Himalaya: studies in religion, history and culture, vol. 2. Gangtok: Namgyal Institute of Tibetology; 2011. p. 127–42.Google Scholar
- Government of India. The census of India. New Delhi: Ministry of Home Affairs; 2011.Google Scholar
- Singh KS. Sikkim. In: Dhamala RR, Rai CD, Dutta MS, Ghatak S, editors. People of India, vol. 24. Kolkata: Anthropological Survey of India; 1993.Google Scholar
- Balikci A. Lamas, shamans and ancestors: village religion in Sikkim. Leiden: Koninklijke Brill; 2008.View ArticleGoogle Scholar
- Government of Sikkim. Sikkim human development report. New Delhi: Routledge; 2015.Google Scholar
- Tshering SD. Socio-economic census of Sikkim: religious aspects. In: Balikci-Denjongpa A, McKay A, editors. Buddhist Himalaya: studies in religion, history and culture, vol. 2. Gangtok: Namgyal Institute of Tibetology; 2011. p. 233–44.Google Scholar
- Government of India. Linguistic survey of India: Sikkim. New Delhi: Office of the Registrar General, Language Division; 2015.Google Scholar
- Wikramanayake E, Dinerstein E, Loucks CJ. Terrestrial ecoregions of the Indo-Pacific: a conservation assessment. Washington: Island Press; 2001.Google Scholar
- ENVIS. Medicinal plants of Sikkim. Gangtok: Government of Sikkim; 2016. http://envis.frlht.org/sikkim/.Google Scholar
- Botanical Survey of India. NELUMBO. 2016; http://bsi.gov.in/DetailsArchive/9_5_NOTICEINVITINGFOREXPRESSIONONTSTFORONLINEJOURNALMANAGEMENTSYSTEMINNELUMBOANDAKINGFNELUMBOASE-JOURNAL.aspx.
- The Johns Hopkins University Press and The Milton S. Eisenhower Library. Project Muse. 2016; https://muse.jhu.edu/.
- Sharma S, Pandit M. A new species of Panax L. (Araliaceae) from Sikkim Himalaya, India. Syst Bot. 2009; doi:10.1600/036364409788606235.
- Panda S. Gaultheria stapfiana (Ericaceae), a species to be recognized: insights from morphology, lead anatomy and pollen morphology. Phytotaxa. 2012.Google Scholar
- eFloras. Annotated checklist of the flowering plants of Nepal. 2015; http://efloras.org/flora_page.aspx?flora_id=110.
- Encyclopedia of Life. EOL. 2016; http://www.eol.org/.
- Global Biodiversity Information Facility. GBIF. 2016; http://www.gbif.org/.
- The Plant List. 2016. The plant list. V.1.1. 2016; http://www.theplantlist.org/.
- World Health Organization. International Classification of Diseases (ICD). 2016; URL: http://www.who.int/classifications/icd/en/.
- da Costa Quinteiro MM, Tamashiro AMG, Santos MG, Pinto LJS, de Morales MG. Inventory and implications of plant use for environmental conservation in Visconde de Maua, Serra da Mantiqueira, Brazil. Ethnobot Res Appl. 2015.Google Scholar
- Saklani A, Upreti D. Folk uses of some lichens in Sikkim. J Ethnopharmacol. 1992; doi:10.1016/0378-8741(92)90038-S.
- Adhikari M, Devkota S, Tiwari R. Ethnomycological knowledge on uses of wild mushrooms in Western and Central Nepal. Our Nature. 2005; doi:10.3126/on.v3i1.329.
- Das K. Mushrooms of Sikkim: Barsey Rhododendron Sanctuary. Gangtok: Sikkim State Biodiversity Board; 2009.Google Scholar
- Borah TR, Rahman H. Mushrooms in biodiversity and food security of Sikkim. In: Arrawatia ML, Tambe S, editors. Biodiversity of Sikkim: exploring and conserving a global hotspot. Gangtok: Department of Information and Public Relations, Government of Sikkim; 2011. p. 29–42.Google Scholar
- Dash SS, Singh P. Trees of Sikkim. In: Arrawatia ML, Tambe S, editors. Biodiversity of Sikkim: exploring and conserving a global hotspot. Gangtok: Department of Information and Public Relations, Government of Sikkim; 2011. p. 89–125.Google Scholar
- Sinha S, Biswas M. Evaluation of antibacterial activity of some lichen from Ravangla, Sikkim, India. Int J Pharma Biosci. 2011;2(4):23–8.Google Scholar
- Sinha GP, Ram TAM. 2011. Lichen diversity in Sikkim. In: Arrawatia ML, Tambe S, editors. Biodiversity of Sikkim: exploring and conserving a global hotspot. Department of Information and Public Relations, Government of Sikkim; 2011. p. 13-28.Google Scholar
- Tamang DK, Dhakal D, Gurung S, Sharma NP, Shrestha DG. Bamboo diversity, distribution patterns and its uses in Sikkim (India) Himalaya. 2013;3(2):1-6.Google Scholar
- Devkota S. Distribution and status of highland mushrooms: a study from Dolpa, Nepal. J Nat Hist Museum. 2008;23:51–9.Google Scholar
- Baniya CB, Solhoy T, Gauslaa Y, Palmer MW. The elevation gradient of lichen species richness in Nepal. Lichenol. 2010; doi:10.1017/S0024282909008627.
- Aryal HP, Budathokio U. Macro-fungi of Karhiya community forest, Western Terai, Nepal. Nepal J Biosci. 2013;2:93–7.Google Scholar
- International Union for the Conservation of Nature. The IUCN Red List of Threatened Species. Version 2015 − 4. 2016; http://iucnredlist.org/.
- Government of Sikkim. Medicinal plant species of conservation concern identified for Sikkim (SK). Gangtok: ENVIS Centre on Conservation of Medicinal Plants. 2014; http://envis.frlht.org/documents/sikkim-medicinal-plants-2014.pdf.
- Gurung B. The medicinal plants of the Sikkim Himalaya. Chakung: Maples; 2008.Google Scholar
- Google. Sikkim. Google Earth Pro. V 7.1. 2015; URL: http://www.google.com/earth/download/gep/agree.html.
- Environmental Systems Research Institute. ArcGIS Desktop. Version 9.3. Redlands, California; 2011.Google Scholar
- Dhar U, Rawal RS, Upreti J. Setting priorities for conservation of medicinal plants: a case study in Indian Himalaya. Biol Conserv. 2000; doi:10.1016/S0006-3207(00)00010-0.
- de Oliveira RLC, Lin Neto EMF, Araújo EL, Albuquerque UP. Conservation priorities and population structure of woody medicinal plants in an area of Caatinga vegetation (Pernambuco State, NE Brazil). Environ Monit Assess. 2007; doi:10.1007/s10661-006-9528-7.
- Sekar KC. 2012. Invasive alien plants of Indian Himalayan region: diversity and implication. Am J Plant Sci. 2012; doi:10.4236/ajps.2012.3202.
- Gaikwad J, Wilson PD, Ranganathan S. 2011. Ecological niche modeling of customary medicinal plant species used by Australian Aborigines to identify species-rich and culturally valuable areas for conservation. Ecol Model; doi:10.1016/j.ecolmodel.2011.07.005.
- Balram S, Dragićević S, Meredith T. A collaborative GIS method for integrating local and technical knowledge in establishing biodiversity conservation priorities. Biodivers Conserv. 2004; doi:10.1023/B:BIOC.0000018152.11643.9c.
- Millette TL, Tuladhar AR, Kasperson RE, Turner BL II. The use and limits of remote sensing for analyzing environmental and social change in the Himalayan middle mountains of Nepal. Global Environ Change. 1995; doi:10.1016/09593780(95)00071-U.
- Zainiewski AE, Lehmann A, Overton JMcC. Predicting species spatial distributions using presence-only data: a case study of native New Zealand ferns. Ecol Model. 2003; doi:10.1016/S0304-3800(02)00199-0≥.
- Campbell A. Note on the Lepchas of Sikkim, with a vocabulary of their language. J Asian Soc Bengal. 1840;9:379–93.Google Scholar
- Hooker JD. The Rhododendrons of the Sikkim-Himalaya. London: Bentham and Reeve; 1849.Google Scholar
- Hooker JD. Himalayan journals: notes of a naturalist in Bengal, the Sikkim and Nepal Himalayas, the Khasia Mountains, etc. London: John Murray; 1855.Google Scholar
- Basu K. Common medicinal plants of Darjeeling and Sikkim. Kolkata: West Bengal Press; 1956.Google Scholar
- Biswas K. Common medicinal plants of Darjeeling and the Sikkim Himalayas. Alipore: West Bengal Government Press; 1956.Google Scholar
- Vallejo-Ramos M, Moreno-Calles AI, Casas A. TEK and biodiversity management in agroforestry systems of different socio-ecological contexts of the Tehuacan Valley. J Ethnobiol Ethnomed. 2016; doi:10.1186/s13002-016-0102-2.
- Tamsang KP. Glossary of Lepcha medicinal plants. Kalimpong: Mani Printing Press; 2004.Google Scholar
- Acharya BK, Chettri B, Vijayan L. Indigenous knowledge of Lepcha community for monitoring and conservation of birds. Indian J Tradit Knowl. 2009;8(1):65–9.Google Scholar
- Chettri N, Sharma E. 2009. A scientific assessment of traditional knowledge on firewood and fodder values in Sikkim, India. Forest Ecology and Management. 2009; doi:10.1016/j.foreco.2009.02.002.
- Dash SS. Useful plants of Kabi Sacred Grove, Sikkim. NELUMBO. 2007.Google Scholar
- Bhasin V. Ecology, culture, and change: tribals of Sikkim Himalaya. New Delhi: Inter-India Publications; 1989.Google Scholar
- Bhasin V, Srivastava VK. Ecology and culture of the Bhutias of North Sikkim. J Human Ecol. 1990;1(3):277–85.Google Scholar
- Bhasin V. Medical anthropology: healing practices in contemporary Sikkim. In: Bhasin V, Bhasin M, editors. Anthropology today: trends, scope and applications, vol. 3. New Delhi: Kamala-Raj Enterprises; 2007. p. 59–94.Google Scholar
- Lachungpa U. Indigenous lifestyles and biodiversity conservation issues in North Sikkim. Indian J Tradit Knowl. 2009; URL: http://www.nopr.niscair.res.in/bitstream/123456789/…/IJTK8(1)51-55.pdf.
- Bhasin V. Pastoralists of Himalayas. J Biodivers. 2013;4(2):83–113.Google Scholar
- Sundriyal M, Rai LK. Wild edible plants of the Sikkim Himalaya. J Hill Res. 1996;9(2):267–78.Google Scholar
- Sundriyal M, Sundriyal R. Underutilized edible plants of the Sikkim Himalaya: need for domestication. Cur Sci. 2003; URL: http://www.iisc.ernet.in/currsci/sep252003/731.pdf.
- Sundriyal M, Sundriyal R. Wild edible plants of the Sikkim Himalaya: marketing, value addition and implications for management. Econ Bot. 2004; doi:10.1163/0013-0001(2004)058.
- Avasthe RK, Kumar A, Rahman H. Edible horticultural crop diversity in Sikkim Himalaya. In: Sikkim biodiversity: significance and sustainability. Gangtok: Sikkim State Council of Science and Technology; 2012. p. 20–32.Google Scholar
- Sundriyal M, Sundriyal R, Sharma E. Dietary use of wild plant resources in the Sikkim Himalaya, India. Econ Bot. 2004;58(4):626–38.View ArticleGoogle Scholar
- Sundriyal M, Sundriyal R, Sharma E, Purohit A. Wild edibles and other useful plants from the Sikkim Himalaya, India. Oecol Mont. 1998;7(1-2):43–54.Google Scholar
- Dash SS, Maiti A, Rai SK. Traditional uses of plants among the urban population of Gangtok, Sikkim. J Econ Taxon Bot. 2003;27(1):317–24.Google Scholar
- Tamang B, Tamang J. Traditional knowledge of bio-preservation of perishable vegetable and bamboo shoots in Northeast India as food resources. Ind J of Tradit Knowl. 2009; URL: http://www.niscair.res.in/sciencecommunication/researchjournals/rejour/ijtk/Fulltextsearh/2009/January2009/IJTK-Vol8(1)-January2009-pp89-95.htm.
- Tamang JP, Thapa S, Tamang N, Rai B. Indigenous fermented food beverages of Darjeeling hills and Sikkim: process and product characterization. J Hill Res. 1996;9(2):401–11.Google Scholar
- Yonzan H, Tamang JP. Consumption pattern of traditional fermented foods in the Sikkim Himalaya. J Hill Res. 1998;11(1):112–5.Google Scholar
- Singh HB, Jain A. Ethnobotanical observation on the preparation of Millet beer in Sikkim state, India. J Econ Taxon Bot. 1999;23(2):577–9.Google Scholar
- Sundriyal M, Sundriyal R. Seedling growth and survival of selected wild edible fruit species of the Sikkim Himalaya, India. Acta Oecol. 2005; doi:10.1016/j.actao.2005.02.003.
- Chheri N, Sharma E. Non-timber forest produce: utilization, distribution, and status in the Khangchendzonga Biosphere Reserve, Sikkim, India. In: Arrawatia ML, Tambe S, editors. Biodiversity of Sikkim: exploring and conserving a global hotspot. Department of Information and Public Relations, Government of Sikkim; 2011. p. 165-83.Google Scholar
- Chhetri N, Sharma E, Lama S. Non-timber forest produces utilization, distribution, and status in a trekking corridor of Sikkim, India. Lyonia. 2005; URL: http://www.lyonia.org/downloadPDF.php?pdfID=143.
- Das K. Promising wild mushrooms from Sikkim Himalaya with ethnomycological significance and potentialities. In: Kumar S, editor. Ethnobotanical studies in India. New Delhi: Deep Publications; 2014. p. 168–86.Google Scholar
- Rai PC. The practice of traditional healing and the healers of Ribdi-Bhareng. Gangtok: Village Health Association of Sikkim; 2010.Google Scholar
- Acharjee M, Roy D. Rong ichthyological knowledge: a leeway. King Gaeboo Aachyok. 2013;4:25–34.Google Scholar
- Tamang P. Ichthyo-faunal diversities of Sikkim: Taxonomic list, fisheries trend, and conservation exigencies. In: Sikkim biodiversity: significance and sustainability. Gangtok: Sikkim State Council of Science and Technology; 2012. p. 170–9.Google Scholar
- Rai LK, Prasad P, Sharma E. Conservation threats to some important medicinal plants of the Sikkim Himalaya. Biol Conserv; 2010. doi:10.1016/S0006-3207(99)00116-0.
- Chettri A, Barik SK. Assessing ethnobotanical value and threat status of Tetrastigma rumicispermum (Lawson) Planch, a lesser known liana species of Khangchendzonga Biosphere Reserve, Sikkim. Ind J Trad Know. 2013; URL: http://188.8.131.52/handle/123456789/16852.
- Chhetri DR. Ethnomedicinal plants of the Khangchendzonga National Park, Sikkim, India. Ethnobot. 2005; URL: http://184.108.40.206:8080/jspui/handle/1/3649.
- Dhakal RD, Sharma G, Basnet K. Study of community dependence on Menam Wildlife Sanctuary in the Sikkim Himalayas. J Hill Res. 2006;19(1):24–33.Google Scholar
- Lepcha S, Das A. Ethno-medico-botanical exploration along the international borders to Tibet Autonomous Region of China and the kingdom of Bhutan with special reference to the Pangolakha Wildlife Sanctuary, East Sikkim. In: Ghosh C, Das AP, editors. Recent studies in biodiversity and traditional knowledge in India. Malda: Gour College Press; 2011. p. 257–70.Google Scholar
- Shrestha B, Basnett H, Prosanta P. Herbal remedies practiced by traditional practitioners of Nepali tribe in Sikkim. Uni J Pharma Sci Res. 2015; http://ujpsr.com/sites/default/files/articles/UJPSRMNSC-1%281%29.pdf.
- Borah TR, Avasthe R, Helim R. Large Cardamom (Amomum sublatum): a traditional cash crop of Sikkim. Asian Ag Hist. 2012;16(3):271–7.Google Scholar
- Kumar S, Raju DCS. Large Cardamom and its wild relatives in Sikkim Himalayas. J Hill Res. 1998;2(2):375–79.Google Scholar
- Kumar S, Singh P, Singh V. Ethnobotanical aspects of some arboreal and arborescent taxa of Sikkim. In: Gupta B, editor. Higher plants of the Indian sub-continent. New Delhi: BSMPS; 1994. p. 164–6.Google Scholar
- Charnley S, Fischer AP, Jones ET. Integrating traditional and local ecological knowledge into forest biodiversity conservation in the Pacific Northwest. Forest Ecol Mang. 2007; doi:10.1016/j.foreco.2007.03.047.
- Bourdet-Sabatier S, Balikci-Denjongpa A. The Dzumsa of Lachen: an example of a Sikkimese political institution. Bull Tibetol. 2004; http://www.thlib.org/static/reprints/bot/bot_2004_01_04.pdf.