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  • Open Access

Diversity, knowledge, and valuation of plants used as fermentation starters for traditional glutinous rice wine by Dong communities in Southeast Guizhou, China

Journal of Ethnobiology and Ethnomedicine201915:20

https://doi.org/10.1186/s13002-019-0299-y

  • Received: 1 January 2019
  • Accepted: 5 April 2019
  • Published:

Abstract

Background

Beverages prepared by fermenting plants have a long history of use for medicinal, social, and ritualistic purposes around the world. Socio-linguistic groups throughout China have traditionally used plants as fermentation starters (or koji) for brewing traditional rice wine. The objective of this study was to evaluate traditional knowledge, diversity, and values regarding plants used as starters for brewing glutinous rice wine in the Dong communities in the Guizhou Province of China, an area of rich biological and cultural diversity.

Methods

Semi-structured interviews were administered for collecting ethnobotanical data on plants used as starters for brewing glutinous rice wine in Dong communities. Field work was carried out in three communities in Guizhou Province from September 2017 to July 2018. A total of 217 informants were interviewed from the villages.

Results

A total of 60 plant species were identified to be used as starters for brewing glutinous rice wine, belonging to 58 genera in 36 families. Asteraceae and Rosaceae are the most represented botanical families for use as a fermentation starter for rice wine with 6 species respectively, followed by Lamiaceae (4 species); Asparagaceae, Menispermaceae, and Polygonaceae (3 species respectively); and Lardizabalaceae, Leguminosae, Moraceae, Poaceae, and Rubiaceae (2 species, respectively). The other botanical families were represented by one species each. The species used for fermentation starters consist of herbs (60.0%), shrubs (23.3%), climbers (10.0%), and trees (6.7%). The parts used include the root (21.7%), leaf (20.0%), and the whole plant (16.7%). Findings indicate a significant relationship between knowledge of plants used as fermentation starters with age (P value < 0.001) and educational status (P value = 0.004) but not with gender (P value = 0.179) and occupation (P value = 0.059). The species that are most used by informants include Pueraria lobata var. montana (Lour.) van der Maesen (UV = 1.74; Leguminosae), Actinidia eriantha Benth. (UV = 1.51; Actinidiaceae), Oryza sativa L. var. glutinosa Matsum (UV = 1.5; Poaceae).

Conclusion

This study highlights that while most of the Dong informants continue to use a diverse range of plants as a fermentation starter for brewing glutinous rice wine, knowledge of these plants is being lost by the younger generations. Documentation of traditional ethnobotanical knowledge and outreach is thus needed to conserve biocultural diversity in the rural Dong communities in southern China.

Keywords

  • Ethnobotany
  • Traditional ecological knowledge
  • Rice wine
  • Fermented beverage
  • Fermentation starters

Background

Fermented beverages have a long history of preparation and use globally for medicinal, social, and ritualistic purposes [14]. In China, different socio-linguistic groups in regions throughout the country have developed their own characteristic fermented beverages that are associated with cultural identity and social aspects of communities [3, 4]. For example, Guyuelongshan is a rice wine from Shaoxing in Zhejiang Province, Hejiu is a rice wine from Shanghai, and koumiss is a Mongolian liquor [5]. In addition, Tibetan communities prepare barley wine and there are many types of sweet rice wine from southwestern China including “nuomi” that are consumed during weddings, hospitality, funerals, ancestor worship, and other ceremonies [5].

Rice wine is among the most common and oldest fermented beverages in China. It is fermented using a fermentation starter, also known as koji (or jiuqu in Mandarin) [6]. Koji can be made with staple crops such as wheat, rice, millet, and maize that consist of microorganisms that support the fermentation process [7]. For example, communities in Shaoxing prepare koji as a raw material for rice wine from wheat that harbors many microorganisms including Absidia, Acetobacteria, Aspergillus, Bacillus, Mucor, Lactobacillus, and Rhizopus [8]. Some of these microorganisms are also used as single strains for the industrial manufacture of rice wine. Zhang et al. [9] highlighted that Aspergillus oryzae SU16, as a single strain, could be used in the production of koji.

In addition to common staple grains such as wheat, rice, millet, and maize for the preparation of fermentation starters, indigenous groups in mountainous regions of China have a long history of using a wide diversity of local plants for making koji. We previously documented a total of 103 species in 57 botanical families of wild plants that are traditionally used as starters for preparing fermented beverages by Shui communities in southwestern China [4]. The Dong are a socio-linguistic group (also known as the Kam) of southeast Guizhou that also have a long history of using koji for producing glutinous wine as a source of livelihood. Our previous studies demonstrate that the Dong people cultivate many varieties of glutinous rice [10, 11] which they use as their staple food. However, there remains a lack of documentation regarding the plants used as fermentation starters by Dong communities. This study seeks to address this knowledge gap by identifying the diversity of plants used as fermentation starters (koji) by Dong communities and associated knowledge and values. Findings have the potential to inform the conservation of natural resources associated with a culturally-relevant beverage of Dong communities while preserving traditional ecological knowledge.

Methods

Study area

Research was carried out in three Dong villages in Qiandongnan Miao and Dong Autonomous Prefecture in the southeastern part of Guizhou Province (longitude 108°50.3′ E–109°58.5′ E, latitude 25°53.7′ N–26°24.2′ N), located near Hunan and Guangxi provinces. These villages are in the core zone of Dong socio-linguistic group and include Xiaohuang of Congjiang County, Huanggang of Liping County, and Nongwu of Rongjiang County (Fig. 1, Table 1). The three villages have a combined area of 51.22 km2 and are located between 630 and 780 m above sea level. The climate is characterized as subtropical monsoon humid with an annual average temperature of 18.4 °C, an average precipitation of 1200 mm, average sunlight time of 1300 h, and a frost-free period of 310 days per year.
Fig. 1
Fig. 1

Geographic location of the study area: Xiaohuang, Huanggang, and Nongwu in three counties of Congjiang, Liping, and Rongjiang, respectively (Qiandongnan Miao and Dong Autonomous Prefecture, China)

Table 1

Study area (three Dong villages in Qiandongnan Miao and Dong Autonomous Prefecture)

Village name

No. of family

Population

Area (km2)

Altitude (m)

Geographic location

Xiaohuang (Congjiang)

740

3800

16.53

630

25°53.7′ N, 109°58.5′ E

Huanggang (Liping)

325

1629

29.70

780

26°24.2′ N, 109°14.6′ E

Nongwu (Rongjiang)

135

550

4.99

740

25°94.1′ N, 108°50.3′ E

The three study site villages are dominated by members of the Dong and Miao socio-linguistic groups. Traditional rice-fish co-culture system predominates in these villages and integrates with animal husbandry, forestry management, and medicinal plant collection and trade [10, 11]. In this study, we selected to focus on interviewing Dong households because of their longer history of cultivating glutinous rice (Oryza sativa var. glutinosa) compared to the Miao as well as their subsistence lifestyle for procuring food. Glutinous rice wine is a very popular fermented beverage in local communities. The Dong, as many indigenous communities, rely on their environment for a range of wild and cultivated crops for preparing food, beverages, and medicine [12, 13]. The above information indicates that these villages are ideal areas for studying the traditional knowledge of plants used as fermentation starters for traditional glutinous rice wine.

Ethnobotanical data collection

Ethnobotanical surveys were carried out from September 2017 to July 2018. A total of 217 informants (including 126 male and 91 female) were interviewed from the three study sites (Table 2). Semi-structured interviews were carried out using a snowballing approach of meeting Dong community members including in fields, around fish ponds, in canteens, in artisanal workshops, in farmhouses, and in village squares. The semi-structured interviews involved open-ended questions and conversations with informants in the above scenes. The major questions are as follows:
  1. 1.

    Do you know about “Jiuqu” (fermentation starters for brewing traditional glutinous rice wine)?

     
  2. 2.

    Do you know the technology of koji-making?

     
  3. 3.

    If yes, which plants did you choose, and which parts of the plants to make the fermentation starters?

     
  4. 4.

    Where do you usually collect these plants?

     
  5. 5.

    Can you take us to collect these plants? (Field identification or local plant flora).

     
  6. 6.

    Do you know these plant names?

     
  7. 7.

    Can you read these names in Dong language?

     
  8. 8.

    Why do not you choose a commercial “Jiuqu” for brewing traditional glutinous rice wine?

     
  9. 9.

    Would you consider passing this knowledge to your children or other people?

     
  10. 10.

    What other interesting things can you share with us?

     
Table 2

Demographic details of interviewed informants

Category

Subcategory

Number of informants

% of informants

Gender

Male

126

58.06

Female

91

41.94

Age

20–40

22

10.14

40–60

117

53.92

60 and older

78

35.94

Education status

Illiterate

152

70.05

Primary

44

20.28

Secondary

16

7.37

Higher

5

2.30

Occupation

Farmer

133

61.29

Migrant workers

71

32.72

Local officials

13

5.99

Knowledge about koji-making plants

Yes

193

88.94

No

24

11.06

Interviews were carried out in either the Dong language with the assistance of a local Dong translator (Fig. 2) or in simplified Mandarin.
Fig. 2
Fig. 2

Indigenous knowledge of traditional glutinous rice wine koji-making plants: a A local guide to helping identification of glutinous rice wine koji-making plants. b One of face-to-face interview. c The koji for brewing glutinous rice liquor/wine. d Glutinous rice wine made from koji

In the local area, people with primary and higher education tend to go out to work as migrant workers in non-agriculture times, and those with higher education have the opportunity to find permanent jobs in the provincial and prefectural capital cities, or county towns nearby. Interviews in Mandarin were primarily with individuals with primary education or above including migrant workers and local government officials. All interview procedures involved in this study were in accordance with the International Society of Ethnobiology Code of Ethics including procuring prior informed consent before interviews [14]. The demographic characteristics (age, educational status, and occupation) were identified and recorded in all face-to-face interviews (Fig. 2).

In addition to interviews, we carried out participatory observation in the study site communities. Specifically, we focused on observing the process of collecting plants and preparing koji. These observations were supplemented by key informant interviews on the type of plant species. All of the plants mentioned by key informants were identified in the field and collected to prepare voucher specimens. We checked the scientific names of our field collections with The Plant List [15]. Botanical specimens were further examined at the Herbarium of Jishou University, Hunan Province, China. The specimens were assigned voucher numbers and deposited at the Herbarium of Jishou University.

Data analysis

Classical ethnobotanical descriptive statistics were used to summarize ethnobotanical data in Excel 2013. The association between indigenous knowledge of koji-making with participant’s demographic factors including gender, age, educational status, and occupation was tested with Chi-square analysis. Statistical analysis was carried out using SPSS version 20 (SPSS, Chicago) at 5% level of significance (P < 0.05). Use Value (UV) index [16] was calculated to evaluate the botanical species with the greatest use across the study site communities. The UV of each plant mentioned was calculated using the following formula:
$$ \mathrm{UV}=\frac{\sum \mathrm{UP}}{\ n} $$
where UP is the number of uses mentioned by each informant for a given plant use and n is the total number of informants.

Results

Socio-demographic characteristics of respondents

Table 2 describes the demographic characteristics of the 217 study informants. Informants comprised of 58.06% (N = 126) males and 41.94% (N = 91) females. In addition, informants were between the ages of 20 and 96 years (the majority were between 40 and 60 years old). Most of the surveyed respondents (70.05%) are illiterate, and only five (2.30%) of the interviewed respondents had completed higher education (Table 2). The majority of the respondents were farmers (61.29%, N = 133) and migrant workers (32.72%, N = 71), except for a few local government officials (5.99%, N = 13). Most respondents (N = 193; 88.94%) demonstrated average knowledge about koji plants in general (Tables 2 and 3).
Table 3

Knowledge about koji-making plants in relation with gender, age, educational status, and occupation of the respondents

Characteristics

Total number of respondents

Knowledge about koji-making plants

X 2

P value

Yes

No

Gender

   

X2 = 1.807, df = 1

P = 0.179

Male

126

109

17

  

Female

91

84

7

  

Age

   

X2 = 58.668, df = 2

P < 0.001

20–40

22

9

13

  

40–60

117

108

9

  

60 and older

78

76

2

  

Education status

   

X2 = 13.443, df = 3

P = 0.004

None

152

141

11

  

Primary

44

38

6

  

Secondary

16

11

5

  

Higher

5

3

2

  

Occupation

   

X2 = 5.664, df = 2

P = 0.059

Farmers

133

119

14

  

Migrant workers

71

65

6

  

Local officials

13

9

4

  

Diversity of plants used for koji

A total of 60 plant species were documented for preparing koji, belonging to 58 genera and 36 families (Table 4). The most prevalent botanical families were Asteraceae and Rosaceae (N = 6, respectively), followed by Lamiaceae (N = 4); Asparagaceae, Menispermaceae, and Polygonaceae (N = 3, respectively); Lardizabalaceae, Leguminosae, Moraceae, Poaceae, and Rubiaceae (N = 2, respectively); and the other botanical families represented in our collections each consisted of a single species (Table 4).
Table 4

Inventory of plants traditionally used for koji-making in the study area (species are listed alphabetically)

Scientific name

Voucher number

Family name

Dong name

Chinese name

Habit

Part used

UV

Actinidia eriantha Benth.

KJBT0040

Actinidiaceae

Sangp buc dongl

Mao Hua Mi Hou Tao

Shrub

Branch

1.51

Adiantum flabellulatum L.

KJBT0052

Pteridaceae

Kaok naeml

Shan Ye Tie Xian Jue

Herb

Leaf

0.79

Agrimonia pilosa Ledeb

KJBT0029

Rosaceae

Demh Meix Sais

Lu Bian Huang

Herb

Root

0.47

Akebia quinata (Houtt.) Decne.

KJBT0064

Lardizabalaceae

Gueel nyanl bads

Ba Yue Gua

Shrub

Fruit

0.67

Arctium lappa L.

KJBT0027

Asteraceae

Mal kap gueec

Niu Bang

Herb

Aerial part

0.45

Artemisia annua L.

KJBT0019

Asteraceae

Mal yaems sul

He Hao

Herb

Root

1.19

Asarum forbesii Maxim.

KJBT0033

Aristolochiaceae

Naos max tic

Ma Ti Xiang

Herb

Leaf

0.77

Asparagus cochinchinensis (Lour.) Merr.

KJBT0050

Asparagaceae

Sangp begs sangp laox

Tian Men Dong

Herb

Root

0.56

Bauhinia brachycarpa Wall. ex Benth.

KJBT0059

Leguminosae

Jaol bav

Ye Guan Men

Shrub

Root

0.47

Cayratia trifolia (L.) Domin

KJBT0023

Vitaceae

Jaol meixguv

San Ye Wu Lian Mei

Shrub

Fruit

1.25

Cirsium japonicum DC.

KJBT0044

Asteraceae

Mal sax bav laox

Da Ji

Herb

Root

0.31

Clerodendrum cyrtophyllum Turcz.

KJBT0009

Lamiaceae

Bav sup geel kuenp

Da Qing Ye

Shrub

Aerial part

0.44

Codonopsis pilosula

KJBT0011

Campanulaceae

Demh Gaams Yous

Dang Shen

Climber

Root

0.40

Cunninghamia lanceolata (Lamb.) Hook.

KJBT0047

Taxodiaceae

Meix beens

Sha Mu Ye

Tree

Leaf

1.24

Cyclea racemosa Oliv.

KJBT0002

Menispermaceae

Jaol enl sup dangl

Lun Huan Teng

Herb

Branch

0.79

Diospyros cathayensis Steward

KJBT0048

Ebenaceae

Meix bav minc

Shi Zi Ye

Tree

Leaf

0.86

Elaeagnus pungens Thunb.

KJBT0051

Elaeagnaceae

Demh nyox senc

Hu Tui Zi

Shrub

Aerial part

0.78

Fallopia multiflora (Thunb.) Harald.

KJBT0018

Polygonaceae

Jaol maenc yeex

He Shou Wu

Climber

Root

0.28

Ficus pumila L.

KJBT0006

Moraceae

Jaol liangc fenx

Cheng Tuo Guo

Tree

Leaf

0.27

Ficus tikoua Bur.

KJBT0013

Moraceae

Jaol demh xeens

Di Gua Teng

Climber

Whole plant

0.47

Gardenia jasminoides Ellis

KJBT0022

Rubiaceae

Wap lagx ngoc

Huang Zhi Zi

Shrub

Flower

1.03

Gaultheria leucocarpa Bl. var. crenulata (Kurz) T. Z. Hsu

KJBT0053

Ericaceae

Melx demh miuus

Bai Zhu Shu

Herb

Leaf

1.25

Gentiana rhodantha Franch. ex Hemsl.

KJBT0028

Gentianaceae

Nyangt boy liongc

Long Dan Cao

Herb

Whole plant

1.21

Gerbera piloselloides (L.) Cass.

KJBT0034

Asteraceae

Sangp mal kap gav

Mao Da Ding Cao

Herb

Whole plant

1.00

Geum macrophyllum Willd.

KJBT0030

Rosaceae

Yangh muic naemx

Lu Bian Qing

Herb

Aerial part

0.92

Glochidion puberum (Linn.) Hutch.

KJBT0049

Phyllanthaceae

Meix sonp ponc

Suan Pan Zi

Tree

Fruit

1.07

Gonostegia hirta (Bl.) Miq.

KJBT0038

Urticaceae

Mal kgoux lail

Nuo Mi Tuan

Herb

Whole plant

0.92

Hedera nepalensis var. sinensis (Tobl.) Rehd.

KJBT0005

Araliaceae

Jaol bav yaop

Chang Chun Teng

Shrub

Aerial part

0.40

Houttuynia cordata Thunb.

KJBT0063

Saururaceae

Sangp wadc

Zhe Er Gen

Herb

Root

1.46

Imperata cylindrica (L.) Beauv.

KJBT0003

Poaceae

Sangp nyangt bagx

Bai Mao Gen

Herb

Root

1.12

Kadsura longipedunculata Finet et Gagnep.

KJBT0046

Schisandraceae

Jaol dangl bogl padt

Shan Wu Wei Zi

Shrub

Bark

1.47

Kalimeris indica (L.) Sch.-Bip.

KJBT0032

Asteraceae

Mal langx

Ni Qiu Chuan

Herb

Aerial part

0.76

Leonurus japonicus Houtt.

KJBT0060

Lamiaceae

Mal semp beengc

Yi Mu Cao

Herb

Whole plant

0.96

Ligularia fischeri (Ledeb.) Turcz.

KJBT0042

Asteraceae

Bav dinl max

Ti Ye Tuo Wu

Herb

Branch

0.46

Melastoma dodecandrum Lour.

KJBT0014

Melastomataceae

Mal demh xeens

Di Shen

Shrub

Leaf

0.79

Mentha canadensis

KJBT0043

Lamiaceae

Naos suic yeex

Bo He

Herb

Leaf

1.46

Oryza sativa var. glutinosa Matsum.

KJBT0037

Poaceae

Oux

Nuo He

Herb

Stem

1.50

Paris polyphylla Smith

KJBT0039

Melanthiaceae

Wap bar Yeal

Qi Ye Yi Zhi Hua

Herb

Whole plant

0.55

Polygala sibirica L.

KJBT0017

Polygalaceae

Sangp jeml meec angh

Gua Zi Jin

Herb

Aerial part

0.82

Polygonatum cyrtonema Hua

KJBT0021

Asparagaceae

Xingp mant jenc

Huang Jing

Herb

Root

1.00

Polygonum hydropiper L.

KJBT0026

Polygonaceae

Meix bav

La Liao

Herb

Leaf

1.42

Portulaca oleracea L.

KJBT0016

Portulacaceae

Mal Nguedc

Gua Zi Cai

Herb

Whole plant

1.00

Pteridium aquilinum (L.) Kuhn var. latiusculum (Desv.) Underw. ex Heller

KJBT0062

Dennstaedtiaceae

Kaok

Jue Cai

Herb

Stem

0.92

Pueraria lobata var. montana (Lour.) van der Maesen

KJBT0015

Leguminosae

Sangp nieengv

Ge Teng

Climber

Branch

1.74

Frangula crenata (Siebold & Zucc.) Miq.

KJBT0024

Rhamnaceae

Meix liuucliic

Ku Li Ye

Shrub

Leaf

1.22

Rohdea japonica (Thunb.) Roth

KJBT0054

Asparagaceae

Mal nyinc sup

Wan Nian Qing

Herb

Root

1.12

Rosa laevigata Michx

KJBT0065

Rosaceae

Ongv kuaot

Jin Ying Zi

Shrub

Fruit

1.38

Rosa roxburghii Tratt.

KJBT0007

Rosaceae

Sunl ongv kuaot

Ci Li

Herb

Fruit

1.44

Rubus pluribracteatus L.T.Lu & Boufford.

KJBT0008

Rosaceae

Demh bav daemh gal

Da Hei Mei

Climber

Fruit

1.42

Sanguisorba officinalis L.

KJBT0020

Rosaceae

Sangp lagx lugx yak

Hong Di Yu

Herb

Root

0.81

Sargentodoxa cuneata (Oliv.) Rehd. et Wils.

KJBT0057

Lardizabalaceae

Jaol bogl padt yak mags

Xue Teng

Herb

Branch

1.20

Solanum americanum Mill.

KJBT0025

Solanaceae

Lianh yeex

Ye Hai Jiao

Herb

Fruit

1.29

Stephania cepharantha Hay.

KJBT0045

Menispermaceae

Sunl maenc jinc

Jin Xian Diao Wu Gui

Herb

Root

0.88

Teucrium quadrifarium Buch.-Ham. ex D. Don

KJBT0036

Lamiaceae

Nyangt ous

Niu Wei Cao

Herb

Whole plant

0.46

Thalictrum microgynum Lecoy. ex Oliv.

KJBT0056

Ranunculaceae

Wangc lieenc naemx

Xiao Guo Tang Song Cao

Herb

Whole plant

0.45

Tinospora sagittata Gagnep.

KJBT0004

Menispermaceae

Sangp juc saengc

Qing Niu Dan

Shrub

Leaf

0.47

Uncaria rhynchophylla (Miq.) Miq. ex Havil.

KJBT0010

Rubiaceae

Sangp jaol kgoul daov

Da Ye Gou Teng Ye

Climber

Branch

1.32

Verbena officinalis L.

KJBT0031

Verbenaceae

Nyangt piudt max bieenh

Ma Bian Cao

Herb

Leaf

0.79

Viola philippica Cav.

KJBT0012

Violaceae

Mal mac keip

Di Cao Guo

Herb

Whole plant

0.47

Zanthoxylum bungeanum Maxim.

KJBT0066

Rutaceae

Sangp siul yanl

Hua Jiao

Shrub

Fruit

0.92

Analysis of the life forms of koji-making plants showed that 60.0% of the reported species are herbaceous plants (N = 36), 23.3% are shrubs (N = 14), 10.0% are lianas (10.0%), and 6.7% are trees (N = 4) (Table 4). The root was the most commonly used plant part (21.7%, N = 13 citations), followed by the leaf (20.0%, N = 12), whole plant (16.7%, N = 10), fruit (13.3%, N = 8), aerial part (11.7%, N = 7), branch (10.0%, N = 6), stem (3.3%, N = 2), bark, and flower (1.7%, N = 1, both) (Table 4, Fig. 3).
Fig. 3
Fig. 3

Percentage of koji-making plant parts used

Traditional knowledge on koji-making plants

Results of the Chi-square test showed that there was no significant association between knowledge of the koji-making plants and gender (X2 = 1.807, df = 1, P value = 0.179) and occupation (X2 = 5.664, df = 2, P value = 0.059). However, there was a significant association between knowledge of koji plants with age (X2 = 58.668, df = 2, P value < 0.001) and educational status (X2 = 13.443, df = 3, P value = 0.004) (Table 3). Informants older than 40 years and those with lower educational status were the most knowledgeable regarding plants for making koji (Table 3).

Frequently utilized species

The use values (UV) calculated for this study range from 0.27 to 1.74, with a higher UV indicating the plant was more frequently reported to be used by informants. The plant species most frequently utilized by informants for making koji are Pueraria lobata var. montana (Lour.) van der Maesen (1.74), Actinidia eriantha Benth. (1.51), and Oryza sativa L. var. glutinosa Matsum (1.5). There were 23 other species with a UV value greater than 1 including Kadsura longipedunculata Finet et Gagnep, Houttuynia cordata Thunb., Mentha canadensis L., Rosa roxburghii Tratt, Polygonum pubescens (Meissn.) Steward, Rubus pluribracteatus L.T. Lu & Boufford, Rosa laevigata Michx, Uncaria rhynchophylla (Miq.) Miq. ex Havil, Solanum americanum Mill., Cayratia trifolia (L.) Domin, Gaultheria leucocarpa Bl. var. crenulata (Kurz) T. Z. Hsu, Cunninghamia lanceolata (Lamb.) Hook, Frangula crenata (Siebold & Zucc.) Miq., Gentiana rhodantha Franch. ex Hemsl, Sargentodoxa cuneata (Oliv.) Rehd. et Wils, Artemisia annua L., Imperata cylindrica (L.) Beauv, Rohdea japonica (Thunb.) Roth, Glochidion puberum (L.) Hutch, Gardenia jasminoides Ellis, Gerbera piloselloides (L.) Cass, Polygonatum cyrtonema Hua, and Portulaca oleracea (L.) (Table 4).

Discussion

The technique of using plants as fermentation starters is a prevalent traditional method for preparing many well-known fermented foods and beverages in China [17, 18]. This study highlights the diversity of plants used by Dong communities as fermentation starters for making rice wine as well as associated knowledge and use value based on the most frequently reported plants used for koji. We documented a total of 60 plant species and associated plant parts used by informants in the Dong study site communities as fermentation starters for making glutinous rice wine. Our results further showed that 88.94% of respondents had knowledge about plants used as fermentation starters. This finding indicates the rich indigenous ecological knowledge regarding plants in Dong communities which contributes to sustaining livelihoods and well-being along with biodiversity.

Many informants claimed “People who cannot make glutinous rice wine are not a real Dong people, because drinking and singing become a part of our daily life.” This naive view clearly emphasized the importance of fermented beverages in Dong communities and partially suggested that the koji for brewing glutinous rice wine was widely used in the area. Our results further showed that there was no significant difference in knowledge of koji plants between gender or social occupation. These results suggest that koji plants are generally known by local people irrespective of their gender or job.

An older informant (the old woman in red shirt in Fig. 2b) said “Glutinous rice wine is easy to brew, but making koji is a profound knowledge that young people won’t understand.” This statement has been cross-validated among several other informants. Interestingly, the results of this survey showed a significant association between knowledge of koji plants and the respondent’s age, indicating that elder people have more knowledge about koji plants than young people.

Although our results showed there was a significant negative correlation between the education level of respondents and the traditional knowledge on koji plants they possess, findings from this study are in line with another study that shows that educational status does not contribute to the mastery of traditional ecological knowledge [19]. But we cannot conclude that the education status decreased this traditional knowledge. Because the ratio of educated informants was too small, while education is more or less related to age (the younger people are more educated than older ones). It is worth mentioning that, in the study area, many young community members intend to go to distant cities for higher education from an early age. Thus, their communication with elders about traditional glutinous rice wine koji plants is limited.

The 60 species documented in this study represent a diverse range of botanical genera; specifically, the koji plants belong to 58 genera and 36 families with the dominant families including Asteraceae, Rosaceae, Lamiaceae, Asparagaceae, Menispermaceae, and Polygonaceae. A comparison of findings from this study with other regional surveys on plants used as fermentation highlights how species composition and diversity notably varies on the basis of cultural group. A survey by Hong et al. [4] with the Shui socio-linguistic group, also in Guizhou Province, documented that respondents harvested 103 wild plant species in 88 genera and 57 families used as starters for preparing fermented beverages. The majority of plants belonged to the families Asteraceae, Rosaceae, Fabaceae, Melastomaceae, Moraceae, and Rutaceae. For example, Shui communities have been shown to use 9 species in the Rosaceae as fermentation starters (Agrimonia pilosa Ledeb., Geum aleppicum Jacq., Rosa roxburghii Tratt., Rosa laevigata Michx., Rubus alceaefolius Poir., Rubus corchorifolius L., Rubus ellipticus Sm., Rubus xanthocarpus Bureau & Franch., and Rubus niveus Thunb.) while Dong communities use 6 species in the family for koji (Agrimonia pilosa Ledeb., Geum macrophyllum, Rosa laevigata Michx., Rosa roxburghii Tratt., Rubus pluribracteatus L., and Sanguisorba officinalis L.). This comparison demonstrates the distinctiveness in species composition among different socio-linguistic groups within the same region (Guizhou Province) of China. Through our interviews, we got a general understanding of traditional technology of local starter-making. They roughly mashed the cleaned plants and plant parts with a wooden hammer, then stirred the powder of the glutinous rice shell into the mixture until mixing, and then rubbed or rolled the mixture into a bolus between hands. After wetting the surface of the bolus with water from mountain springs, they put the mixture in a barrel and let it ferment naturally, and then place it in indoors for air drying after the surface of bolus has grown white mold.

At the same time, a comparison of findings from this study with other regional surveys on plants highlights how species composition and diversity may also show convergence between cultural groups. Specifically, the species composition found in this study has notable congruence to the general floristic profile of Miao community reported by Liu et al., which revealed that the Rosaceae, Asteraceae, Poaceae, and Liliaceae were dominant botanical families in Puding, Guizhou Province [20]. The analysis of the community structure of local plants in the study area confirms the rationality of the versatility hypothesis of Gaoue et al. [21]. The traditional practice of plant uses, along with the enhancement of the brewing technology, contributes to the diversity and complexity in the use of koji plants by the Dong.

As species and family level alone are not enough to comprehensively understand the keystone ethnobotanical species of koji plants, a quantitative evaluation method of calculating use values (UV) was applied in this study. UV is a commonly used indicator in the fields of ethnobotany and ethnoecology [15]. The evaluation of UV has the potential to reveal the utilization value of plant species and identify culturally-important plant resources [18]. Findings on UV in Dong communities showed that some parts of plant species had very restricted uses. For example, stems of Pueraria lobata var. montana, Actinidia eriantha, and glutinous rice were not reported in any published ethnobotanical studies as food or food raw materials. Alternatively, we found some koji plants widely reported in the literature as edible wild vegetables or fruits while having limited commercial use in the study area. Examples of these plants include Artemisia annua [22], Elaeagnus pungens [23], Houttuynia cordata [24], Portulaca oleracea [25], Pteridium aquilinum var. latiusculum (bracken fern) [26], Rosa laevigata, and Rosa roxburghii [27]. Additionally, we identified multiple other plants used by study informants for koji that have not been reported for this use in other geographical and sociocultural contexts, including “Naos suic yeex” (Mentha canadensis) and “Sangp siul yanl” (Zanthoxylum bungeanum). Mentha canadensis is a widely used plant to extract essential oil [28] and is also consumed in China for medicinal purposes in treating human diseases and to enhance appetite. The fruit of Zanthoxylum bungeanum is popular as a seasoning and traditional Chinese herbal medicine, and widely distributed in China and some Southeast Asian countries [29].

Conclusion

This study highlights that the majority of Dong informants in the study site communities continue to use a wide diversity of plants as fermentation starters for brewing glutinous rice wine, a tradition that is over a thousand years old. In addition, this study highlights that elders in study site communities continue to have richer traditional ecological knowledge regarding plants used as fermentation starters and that this knowledge is not being transmitted to the younger generation. The most prevalent koji plants reported in this study include Pueraria lobata var. montana (Lour.) van der Maesen stem, Actinidia eriantha Benth., and Oryza sativa var. glutinosa Matsum. stem. Findings of this study can be used to inform programs focused on the preservation of botanical resources used for preparing traditional glutinous rice wine edge. Similar to our findings of dye plants in the Dong area [30], we suggest supporting educational workshops and training focused on transmitting the traditional ecological knowledge of community elders to the younger generation. It is expected that such efforts will not only support the cultural identity of communities through the preservation of knowledge and practices, but will also help conserve surrounding biodiversity that is embedded in traditional ecological knowledge.

Declarations

Acknowledgements

The authors acknowledge the local people in the study area. We also thank Professor Daoying Lan, Jishou University, China, for his critical reading and extensive comments on this manuscript.

Funding

This study was financed by the National Natural Science Foundation of China (31761143001, 31870316 & 31560088), Key Laboratory of Ethnomedicine (Minzu University of China) of Ministry of Education of China (KLEM-ZZ201806), Minzu University of China (Collaborative Innovation Center for Ethnic Minority Development, YLDXXK201819), Ministry of Education of China and State Administration of Foreign Experts Affairs of China (B08044), the Special Funds Project for Central Government Guides Local Science and Technology Development (2018CT5012), Open Programme of Center of Tujia Medical Research in Hunan Province, China (2017-6), and the Research Platform Foundation of Jishou University (JD201605, NLE201708).

Availability of data and materials

The data for this study may be availed upon request.

Authors’ contributions

CLL conceived and designed the study. JWH and RFZ collected the data. QYL performed the statistical analysis. JWH, RFZ, GXC, KGL, and QYL participated in discussions. SA and CLL finalized the manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

We followed ethical guidelines adopted by the International Society of Ethnobiology (2008). Permissions were verbally informed by all participants in this study. All people appeared in Fig. 2 agreed to publish the photos.

Consent for publication

Not applicable

Competing interests

The authors declare that they have no competing interests.

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Authors’ Affiliations

(1)
College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China
(2)
Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, 100081, China
(3)
National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides, Jishou University, Jishou, 416000, Hunan, China
(4)
School of Health Science, Kaili University, Kaili, 556011, Guizhou, China
(5)
The Food and Health Lab, Department of Health and Human Development, Montana State University, Bozeman, MT 59717, USA
(6)
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

References

  1. Sõukand R, Pieroni A, Biró M, Dénes A, Dogan Y, Hajdari A, Kalle R, Reade B, Mustafa B, Nedelcheva A, Quave CL, Łuczaj Ł. An ethnobotanical perspective on traditional fermented plant foods and beverages in eastern Europe. J Ethnopharmacol. 2015;170:284–96. https://doi.org/10.1016/j.jep.2015.05.018.View ArticlePubMedGoogle Scholar
  2. Egea T, Signorini MA, Ongaro L, Rivera D, de Castro CO, Bruschi P. Traditional alcoholic beverages and their value in the local culture of the Alta Valle del Reno, a mountain borderland between Tuscany and Emilia-Romagna (Italy). J Ethnobiol Ethnomed. 2016;12:27. https://doi.org/10.1186/s13002-016-0099-6.View ArticlePubMedPubMed CentralGoogle Scholar
  3. McGovern PE, Zhang J, Tang J, Zhang ZQ, Hall RG, Moreau AR, et al. Fermented beverages of pre-and proto-historic China. Proc Natl Acad Sci U S A. 2004;101(51):17593–8. https://doi.org/10.1073/pnas.0407921102.View ArticlePubMedPubMed CentralGoogle Scholar
  4. Hong LY, Zhuo JX, Lei QY, Zhou JJ, Ahmed S, Wang CY, et al. Ethnobotany of wild plants used for starting fermented beverages in shui communities of Southwest China. J Ethnobiol Ethnomed. 2015;11:42. https://doi.org/10.1186/s13002-015-0028-0.View ArticlePubMedPubMed CentralGoogle Scholar
  5. Shen F, Wu J, Ying Y, Li B, Jiang T. Differentiation of Chinese rice wines from different wineries based on mineral elemental fingerprinting. Food Chem. 2013;141:4026–30. https://doi.org/10.1016/j.foodchem.2013.06.119.View ArticlePubMedGoogle Scholar
  6. Xu J, Wu H, Wang Z; Zheng F, Lu X, Li Z, et al. Microbial dynamics and metabolite changes in Chinese rice wine fermentation from sorghum with different tannin content. Sci Rep-UK. 2018;8(1):4639. https://doi.org/10.1038/s41598-018-23013-1.View ArticleGoogle Scholar
  7. Teramoto Y, Okamoto K, Ueda S. Rice wine brewing with sprouting rice, sprouting rice infected with Aspergillus oryzae and rice koji. J Inst Brew. 2013;99:467–71. https://doi.org/10.1002/j.2050-0416.1993.tb01185.x.View ArticleGoogle Scholar
  8. Hong X, Chen J, Liu L, Wu H, Tan H, Xie G, et al. Metagenomic sequencing reveals the relationship between microbiota composition and quality of Chinese rice wine. Sci Rep-UK. 2016;6:26621. https://doi.org/10.1038/srep26621.View ArticleGoogle Scholar
  9. Zhang B, Guan ZB, Cao Y, Xie GF, Lu J. Secretome of Aspergillus oryzae in Shaoxing rice wine koji. Int J Food Microbiol. 2012;155:113–9. https://doi.org/10.1016/j.ijfoodemicro.2012.01.014.View ArticlePubMedGoogle Scholar
  10. Lei Q, Zhou J, Zhang W, Luo J, Wu K, Long C. Morphological diversity of panicle traits in Kam fragrant glutinous rice ( Oryza sativa ). Genet Resour Crop Evol. 2018;65:775–86. https://doi.org/10.1007/s10722-017-0570-9.View ArticleGoogle Scholar
  11. Lei Q, Zhou J, Luo J, Zhang W, Sun J, Long C. Changes in the numbers of Kam fragrant glutinous rice varieties in the Dong regions of Guizhou Province. Biodivers Sci. 2017;25:990–8. https://doi.org/10.17520/biods.2017119.View ArticleGoogle Scholar
  12. Ulijaszek SJ. Potential seasonal ecological challenge of heat strain among Australian aboriginal people practicing traditional subsistence methods: a computer simulation. Am J Phys Anthropol. 2001;116:236–45. https://doi.org/10.1002/ajpa.1119.View ArticlePubMedGoogle Scholar
  13. Haener MK, Dosman D, Adamowicz WL, Boxall PC. Can stated preference methods be used to value attributes of subsistence hunting by aboriginal peoples? A case study in Northern Saskatchewan. Am J Agr Econ. 2001;83:1334–40. https://doi.org/10.1111/0002-9092.00287.View ArticleGoogle Scholar
  14. International Society of Ethnobiology 2006. International Society of Ethnobiology Code of Ethics (with 2008 additions). http://ethnobiology.net/code-of-ethics/. Accessed 16 May 2017.
  15. The Plant List. http://www.theplantlist.org. Accessed 10 Feb 2019.
  16. Albuquerque UP, Lucena RFP, Monteiro JM, Florentino ATN. Evaluating two quantitative ethnobotanical techniques. Ethnobotany Res App. 2006;4:51–60. https://doi.org/10.17348/era.4.0.51-60.View ArticleGoogle Scholar
  17. Chen S, Xu Y. Effect of ‘wheat Qu’ on the fermentation processes and volatile flavour-active compounds of Chinese rice wine (Huangjiu). J I Brewing. 2013;119:71–7. https://doi.org/10.1002/jib.59.View ArticleGoogle Scholar
  18. Yan YZ, Qian YL, Ji FD, Chen JY, Han BZ. Microbial composition during Chinese soy sauce koji-making based on culture dependent and independent methods. Food Microbiol. 2013;34:189–95. https://doi.org/10.1016/j.fm.2012.12.009.View ArticlePubMedGoogle Scholar
  19. Brosi BJ, Balick MJ, Wolkow R, Lee R, Kostka M, Raynor W, et al. Cultural erosion and biodiversity: canoe-making knowledge in Pohnpei, Micronesia. Conserv Biol. 2007;21:875–9. https://doi.org/10.1111/j.1523-1739.2007.00654.x.View ArticlePubMedGoogle Scholar
  20. Liu YG, Liu CC, Wei YF, Guo K. Species composition and community structure at different vegetation successional stages in Puding, Guizhou Province, China. Chin J Plan Ecolo. 2011;35:1009–18.Google Scholar
  21. Gaoue OG, Coe MA, Bond M, Hart G, Seyler BC, Mcmillen H. Theories and major hypotheses in ethnobotany. Econ Bot. 2017:1–19. https://doi.org/10.17520/biods.2017119.
  22. de Magalhães PM, Dupont I, Hendrickx A, Joly A, Raas T, Dessy S, et al. Anti-inflammatory effect and modulation of cytochrome P450 activities by Artemisia annua tea infusions in human intestinal Caco-2 cells. Food Chem. 2012;134:864–71. https://doi.org/10.1016/j.foodchem.2012.02.195.View ArticleGoogle Scholar
  23. Bijman J, Ruben R, Boekel MV, Tilburg AV, Trienekes J. The role of producer organisations in quality-oriented agrifood chains; an economic organisation perspective. New Phytol. 2007;72:493–6. https://doi.org/10.3920/978-90-8686-600-7.View ArticleGoogle Scholar
  24. Verma RS, Joshi N, Padalia RC, Singh VR, Goswami P, Kumar A, et al. Chemical composition and allelopathic, antibacterial, antifungal, and antiacetylcholinesterase activity of fish-mint (Houttuynia cordata Thunb.) from India. Chem Biodivers. 2017. https://doi.org/10.1002/cbdv.201700189.
  25. Zhu H, Wang Y, Liu Y, Xia Y, Tang T. Analysis of flavonoids in Portulaca oleracea L. by UV–vis spectrophotometry with comparative study on different extraction technologies. Food Anal Method. 2010;3:90–7. https://doi.org/10.1007/s12161-009-9091-2.View ArticleGoogle Scholar
  26. Tan L, Guo LZ, Song LM, Li DL, Liang W, Liu L. First report of Colletotrichum gloeosporioides causing leaf spot on Pteridium aquilinum in China. Plant Dis. 2017;101. https://doi.org/10.1094/pdis-12-16-1848-pdn.
  27. Li X, Cao W, Shen Y, Li N, Dong XP, Wang KJ, et al. Antioxidant compounds from Rosa laevigata fruits. Food Chem. 2012;130:575–80. https://doi.org/10.1016/j.foodchem.2011.07.076.View ArticleGoogle Scholar
  28. Yu X, Liang C, Chen J, Qi X, Liu Y, Li W. The effects of salinity stress on morphological characteristics, mineral nutrient accumulation and essential oil yield and composition in Mentha canadensis L. Sci Hortic Amst. 2015;197:579–83. https://doi.org/10.1016/j.scienta.2015.10.023.View ArticleGoogle Scholar
  29. Zhang Z, Liu J, Peng S, Cao Y, Lu X, Gao X, et al. Zanthoxylum bungeanum pericarp extract prevents dextran sulfate sodium-induced experimental colitis in mice via the regulation of TLR4 and TLR4-related signaling pathways. Int Immunopharmacol. 2016;41:127–35. https://doi.org/10.1016/j.intimp.2016.10.021.View ArticlePubMedGoogle Scholar
  30. Liu YJ, Ahmed S, Liu B, Guo ZY, Huang WJ, Wu XJ, Li SH, Zhou JJ, Lei QY, Long CL. Ethnobotany of dye plants in Dong communities of China. J Ethnobiol Ethnomed. 2014;10:23. https://doi.org/10.1186/1746-4269-10-23.View ArticlePubMedPubMed CentralGoogle Scholar

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